曲格列酮诱导胃癌SGC7901细胞凋亡的研究_医学论文.docVIP

曲格列酮诱导胃癌SGC7901细胞凋亡的研究_医学论文 曲格列酮诱导胃癌SGC7901细胞凋亡的研究_医学论文 作者：于媛 王娟 杨建 谢书阳 焦飞 【摘要】 目的 观察过氧化物酶体增殖激活受体γ(PPAR γ)的配体曲格列酮(troglitazone，TGZ)对胃癌SGC细胞凋亡的影响。方法 体外培养人胃癌SGC细胞，SGC细胞分为对照组和曲格列酮不同浓度(5、10、15、20 μmol/L)加药组，应用流式细胞仪检测曲格列酮不同浓度对胃癌SGC细胞凋亡率的影响RT及Western blot方法观察PPARγ、p53的mRNA及蛋白表达变化。结果 曲格列酮可诱导胃癌SGC细胞凋亡，凋亡率与浓度呈正相关曲格列酮干预后，p53的mRNA及蛋白在胃癌SGC细胞中表达上调，而PPARγ的mRNA及蛋白表达水平无明显改变。结论 曲格列酮依赖激活PPARγ能在体外诱导胃癌细胞凋亡，其机制可能是通过上调抑癌基因p53表达而实现，提示PPARγ可能是胃癌治疗的一个新分子靶点。 【关键词】 曲格列酮过氧化物酶体增殖激活受体γp53胃癌凋亡 　　【Abstract】 Objective To investigate the effect of peroxisome proliferator activated receptor gamma (PPARγ) ligand troglitazone (TGZ) on induction of apoptosis in human gastric adenocarcinoma cell line SGC7901.Methods Human gastric adenocarcinoma cell line SGC7901 was cultured in vitro. SGC7901 cell line was divided into control group and treatment groups with different concentrations of TGZ (5,10,15,20 μmol/L).The changes of apoptosis at each concentration in the SGC7901 were detected by flow cytometry method. The mRNA and protein expression of PPARγ and p53 were investigated by RTPCR and Western blot respectively.Results The effect of troglitazone on the induction of apoptosis in SGC7901 cells was significant with flow cytometry detection. Furthermore, the rate of apoptosis in SGC7901 cells treated with troglitazone at various concentrations showed a dosedependent manner. After treated with troglitazone,the mRNA and protein expression of p53 was upregulated. However, the expression of PPARγ has no significant changes.Conclusion These results indicate that the apoptosis effect of troglitazone is mediated through PPARγ signaling pathway in gastric adenocarcinoma cell in vitro.The mechanism is probably associated with upregulating of the anticancer gene p53.It’s suggested that PPARγ might be a novel therapeutic target for gastric adenocarcinoma. 　　【Key words】 troglitazone,peroxisome proliferator activated receptor gamma,p53,gastric adenocarcinoma,apoptosis 　　肿瘤的发生是一个多因素、多步骤的复杂过程，涉及一系列遗传学改变，包