束花石斛营养器官内部组织构造的探讨_药学论文.docVIP

束花石斛营养器官内部组织构造的探讨_药学论文 束花石斛营养器官内部组织构造的探讨_药学论文 作者：白音，潘春香，包英华，吴考香 【摘要】 目的探讨药用束花石斛的根、茎、叶的内部组织构造;并且，基于药用石斛的药用部位为茎，对其一、二、三年生茎的内部组织构造进行了观察比较，拟在探讨药用石斛茎随着生长发育其内部组织构造的变化，从而为明确药用石斛茎的年限与其药用质量的关系奠定基础。方法根、茎、叶的内部组织构造采用石蜡包埋法，叶片表面构造采用指甲油涂片法。结果基本明确了单子叶植物束花石斛的内部组织构造特征;并且，观察到二年生和三年生茎与一年生相比，表现为叶鞘退化，薄壁细胞排列不规则，且细胞大小不均匀，维管束细胞壁较厚。结论该研究为药用石斛解剖学研究和药用石斛茎的年限与其药用质量关系的研究提供了解剖学依据。 【关键词】 束花石斛;营养器官;内部组织构造 　Abstract：ObjectiveTo explore the anatomical structure of roots, stalks and leaves of Dendrobium chrysanhum.and the growth and development of medicinal Dendrobium stalk with its internal organization structure changes, in order to lay a solid foundation for clarifying the relationship between the stem of Dendrobium medicinal quality and its medicinal life.MethodsThe morphological and anatomical structure of Dendrobium chrysanhum Lam’s roots, stalks and leaves were observed and compared by the method of paraffin-embedded and the structure of leaf surface was observed by the method of sticky nail polish.ResultsFrom this research,we have realized the anatomical features of beam spent dendrobium,which belongs to the monocotyledon.Moreover，the experiment also showed that the Sheath of two-year-old and three-year-old stalks had the phenomenon of obsolescence,and the arrangement of Parenchyma cells was irregular and the size of cells was not uniform.Apart from this,the bundle’s cellwall became thicker.ConclusionThe study provided the anatomical basis for the study of Medicinal Dendrobium anatomy and the relationship between the life of stalks and its medical quality. 　　Key words：Dendrobium chrysanhum;Nutritional organs;Anatomical structure 　　据不完全考察，我国药用石斛有76种[1]。内部组织构造是鉴别药用石斛的重要方法，目前关于药用石斛营养器官内部组织构造的报道很少，只有关于细叶石斛、铁皮石斛几篇报道。而且，中药材组织构造特征是中药质量评价的主要内容之一。研究证明，药用石斛表皮光泽度、断面颜色深浅、质地、维管束和纤维数量等特征均影响中药质量和临床疗效[2]。随着石斛生长发育，上述器官组织和药材质量均发生较大变化[3~5]。因此，从组织构造特征上系统研究药用石斛质量变化及评价是一项很重要的研究课题。所以，本实验对药用束花石斛内部组织构造，以及随着生长发育，其主要利用部分茎的内部组织构造的变化进行了系统观察比较，这对药用石斛质量评价、分类鉴定以及最佳采收期的确定等方面具有重要指导意义。 　　1 材料与方法 　　1.1 材料野生束花石斛Dendrobium chrysa