活化蛋白-1在膳食牛磺酸防护视网膜光损伤中的表达变化_药学论文.docVIP

活化蛋白-1在膳食牛磺酸防护视网膜光损伤中的表达变化_药学论文 活化蛋白-1在膳食牛磺酸防护视网膜光损伤中的表达变化_药学论文 【摘要】 探讨膳食牛磺酸防护视网膜光化学损伤的分子机制。方法 30只Sprague-Dawley大鼠饲以AIN-93标准饲料或补充4％牛磺酸膳食干预15 d，给予（3 000±200）lux的持续光照24 h，形态测量法观察视网膜外核层厚度，电生理法检测视网膜功能，Western blot或免疫组织化学法检测活化蛋白-1（actor protein-1，AP-1）亚单位c-fos/c-jun蛋白表达，荧光定量PCR法检测其mRNA表达。结果 光照能降低视网膜外核层厚度，降低视网膜电图a波和b波的幅度，升高AP-1蛋白和mRNA的表达，4%膳食牛磺酸在转录和翻译水平下调AP-1的表达，有效防护视网膜光化学损伤。结论 转录因子AP-1在膳食牛磺酸防护视网膜光损伤中发挥着重要作用。 【关键词】 活化蛋白-1 牛磺酸 视网膜 光损伤 Abstract：Objective To investigate the underlying molecular mechanism involving in dietary taurine protecting retina from photochemical damage.Methods Thirty Sprague-Dawley rats fed with or without 4% taurine under AIN-93 diet for 15 d were persistently exposed to fluorescent light(3000±200 lux) for 0-24h.The thickness of outer nuclear layer(ONL)of retinas was measured with morphometry.The retinal function was detected with electrophysiological technique.The expression of actor protein-1(AP-1)subunits c-fos/c-jun protein was analyzed with Western blot or immunohistochemical method,and their mRNA expression determined with quantitative real-time PCR(qRT-PCR).Results There was a decrease in thickness of ONL, and in amplitude of a- and b- waves of electroretinogram,an increase in protein and mRNA expression of c-fos/c-jun subunits in retinas of rats exposed to light.4% dietary taurine effectively prevented retinal photochemical damage as changes of morphology and electroretinogram(ERG).Also,the supplementation caused a down-regulation in expression of c-fos/c-jun subunits.Conclusions Transcription factor AP-1 plays a pivotal role in dietary taurine protecting retina from photochemical damage. Key words:actor protein-1;taurine;retina;light damage 视网膜光化学损伤是视网膜光损伤最主要的一种形式，其早期病理表现为视网膜功能减退，视网膜内外节段排列紊乱甚至断裂消失，感光细胞变性丢失，外核层（Outer nuclear layer，ONL）厚度变薄，后期病理表现为视网膜感光细胞凋亡性坏死，视觉功能严重受损甚至失明［1］。针对视网膜光化学损伤的各致伤环节，现已发现钙通道阻滞剂、地塞米松、天然抗氧化剂、自由基清除剂，以及神经营养因子等对光化学损伤有一定的保护作用，然而这种防护作用的效果并不理想［2］。牛磺酸（Taurine）是体内一种游离的条件必需氨基酸，在视网膜尤其是感光细胞和Muller细胞内含量极为丰富，在视网膜生长发育、维持正常视功能等方面发