淫羊藿总黄酮对MPP+诱导MES23.5细胞损伤保护作用_医学论文.docVIP

淫羊藿总黄酮对MPP+诱导MES23.5细胞损伤保护作用_医学论文 淫羊藿总黄酮对MPP+诱导MES23.5细胞损伤保护作用_医学论文 作者：徐爱丽 张阳 周丽苹 陈文芳【摘要】 目的探讨淫羊藿总黄酮对1-甲基-4-苯吡啶离子(MPP+)诱导的MES23.5细胞损伤的保护作用。方法MES23.5细胞常规培养，淫羊藿总黄酮预处理24 h，再用MPP+和淫羊藿总黄酮共同处理细胞24 h，MTT法检测细胞的存活率，实时反转录聚合酶链反应(real time RT-PCR)观察bcl-2和bax基因的表达情况。结果MPP+可剂量依赖性地损伤MES23.5细胞(F=18.38，0.001)淫羊藿总黄酮预处理可明显对抗MPP+的毒性作用 (F=44.36，0.001)MPP+对bcl-2基因表达没有明显影响，但可明显增加bax基因的表达(F=10.92，0.05)淫羊藿总黄酮预处理可明显增加bcl-2基因的表达(F=15.76,0.01)，并可逆转bax基因表达的增高(F=10.92，0.05)。结论淫羊藿总黄酮可明显对抗MPP+对MES23.5神经细胞的损伤，其作用机制可能与抗凋亡有关。 【关键词】 淫羊藿总黄酮1-甲基-4-苯吡啶离子细胞凋亡MES23.5细胞 　[ABSTRACT]ObjectiveTo study the protection of total flavonoid of Herba Epimedii (TFHE) against 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity.MethodsMES23.5 cells were cultured as routine and pretreated with TFHE for24 h, and then co-treated with MPP+ and TFHE for 24 h. The survival of the cells was observed by MTT method. The real time RT-PCR was used to detect the gene expressions of bcl-2 and bax.ResultsMPP+ damaged MES23.5 cells as a dose-dependent manner (F=18.38,0.001). Pretreatment with TFHE could obviously against toxic action of MPP+ (F=44.36,0.001). MPP+ had no significant effect on bcl-2 gene expression, but increased the gene expression of bax (F=10.92,0.05). Pretreatment of TFHE increased the bcl-2 gene expression (F=15.76,0.01) and reversed the increase of bax gene expression(F=10.92,0.05).ConclusionTotal flavonoid of Herba Epimedii has an antagonistic action on MES23.5 cells damage induced by MPP+, of which, the mechanism may be related to its anti-apoptosis effects. 　　[KEY WORDS]total flavonoid of Herba Epimedii 1-methyl-4-phenylpyridinium apoptosis MES23.5 cells 　　帕金森病(PD)是一种常见的中枢神经系统退行性疾病，PD的主要病理改变是中脑黑质致密带(SNpc)多巴胺能神经元进行性脱失并伴发黑质纹状体轴突末梢多巴胺耗竭[1-2]。PD的病因迄今未明，发病机制十分复杂，可能有多种因素参与，其中氧化应激和细胞凋亡在PD的发病中具有重要的作用。目前，尚无成熟有效的药物或方法能阻止或逆转PD病情的发展。因此，探索并研制开发有效的防治PD的药物成为当今医药界研究的热点。淫羊藿 (Herba Epimedii)，又名仙灵脾，为我国传统的补肾壮阳中药，历代本草药典均有记载。淫羊藿主要化学成分为黄酮类化合物、木脂素及生物碱，其中黄酮类化合物是淫羊藿主要的药理活性组分。研究表明，淫羊藿具有多方面的药理作用，主要集中在免疫系统、生殖系统、骨骼系统、核酸代谢、心脑血管系统及抗衰老等方