电离辐射对胶质瘤C6细胞垂体瘤转化基因表达的影响_医学论文.docVIP

电离辐射对胶质瘤C6细胞垂体瘤转化基因表达的影响_医学论文 电离辐射对胶质瘤C6细胞垂体瘤转化基因表达的影响_医学论文 作者：姜平 戴如飞 蔡军 刘宁 骆慧 闫志海 阎超 【摘要】 目的 探讨电离辐射对胶质瘤C6细胞垂体瘤转化基因（pituitary tumor transforming gene， PTTG）表达的影响。方法 胶质瘤C6细胞分为4组（n=6）：A组（空白对照组）、B组（2 Gy剂量X射线组）、C组（4 Gy剂量X射线组）、D 组（6 Gy剂量X射线组），X射线照射后12 h，逆转录聚合酶链反应（RT-PCR）检测PTTG mRNA的表达,免疫细胞化学检测其蛋白表达。结果 PTTG mRNA在X射线处理组均降低，随着X射线剂量的增加，其表达降低越明显，A、B、C、D 4组的表达分别为 1.910±0.141、 1.398±0.157、1.010±0.133、0.743±0.092，各组间差异均有统计学意义( 0.01)。PTTG蛋白积分光密度 (integrated optical density，IOD) 在X射线处理组均降低，随着X射线剂量的增加，其表达降低越明显，A、B、C、D 4组的IOD分别为1.840±0.089、1.395±0.139、0.893±0.125、 0.597±0.104，各组间差异均有统计学意义( 0.01)。结论 电离辐射可以剂量依赖的方式降低胶质瘤C6细胞PTTG mRNA及其蛋白的表达。 【关键词】 胶质瘤；电离辐射；垂体瘤转化基因 Abstract: Objective To investigate the effect of ionizing radiation on the expression of pituitary tumor transforming gene (PTTG) in glioma C6 cells. Methods Glioma C6 cells were divided into 4 groups (n=6): group A (control group), group B (2 Gy doses of X-ray group), group C (4 Gy doses of X-ray group), group D (6 Gy doses of X-ray group). The expression of PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) 12 hours after X-ray irradiation and the expression of PTTG protein was detected by immunostaining assay using streptavidin-peroxidase (SP) method. Results The expression of PTTG mRNA decreased in X-ray treatment groups, which was positively correlated with the increase of X-ray doses. The expression of PTTG mRNA in groups A, B, C and D were 1.910±0.141, 1.398±0.157, 1.010±0.133, 0.743±0.092, respectively. There were significant differences between these groups (0.01). The expression of PTTG protein decreased in X-ray treatment groups, which was also positively correlated with the increase of X-ray doses. The integrated optical density (IOD) of PTTG protein in groups A, B, C and D were 1.840±0.089, 1.395±0.139, 0.893±0.125, 0.597±0.104, respectively. There were significant differences between these groups (0.01). Conclusion Ionizing radiation can decrease the expression of PTTG mRNA and