电针对力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响_医学论文.docVIP

电针对力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响_医学论文 电针对力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响_医学论文 【摘要】 目的 研究电针刺激对一次性力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响，初步探讨电针刺激提高运动能力、延缓运动性疲劳发生的分子生物学机理。方法 40只SD雄性大鼠分为4组（对照组、模型组、力竭组、电针组），对照组大鼠常规饲养3周后处死取材；其他三组进行3周的游泳训练，模型组大鼠在游泳训练后处死取材；力竭组大鼠在游泳训练后进行一次性力竭游泳后处死取材；电针组大鼠在游泳训练后进行一次电针刺激，然后进行一次性力竭游泳，力竭游泳后处死取材。记录力竭游泳时间，用RT-PCR方法测定骨骼肌α-肌动蛋白基因表达。结果 电针组的平均力竭游泳时间明显长于力竭组；模型组大鼠的骨骼肌α-肌动蛋白基因表达明显高于对照组和力竭组；电针组的α-肌动蛋白基因表达虽低于模型组，但高于力竭组和对照组。结论 （1）3周游泳训练可以提高大鼠骨骼肌α-肌动蛋白的基因表达；（2）电针刺激有助于促进力竭运动大鼠的骨骼肌α-肌动蛋白基因的表达，延长大鼠力竭游泳时间，提高大鼠的运动耐力，延缓运动性疲劳的发生。 【关键词】 电针；肌动蛋白；力竭运动 Effects of electroacupuncture on expression of α-actin mRNA induced by exhausted exercise in skeletal muscles of rats 【Abstract】 Objective To observe the effects of electroacupuncture on expression of α-actin mRNA induced by exhausted exercise in skeletal muscles of rats, and explore the molecular biological mechanisms of electroacupuncture in delaying the occurrence of exercise-induced fatigue.Methods Forty male SD rats were randomly divided into four groups（control group model group exhausted exercise group electroacupuncture group）. Control group was killed after raising for 3 weeks other 3 groups were trained to swimming for 3 weeks, then model group was killed and electroacupuncture group were treated with electroacupunctrue before exhausted exercise while exhausted exercise group attended exhausted exercise directly. Then the exhausted time was noted, and the expression of α-actin was tested by RT-PCR.Results The mean time of exhausted swimming in electroacupuncture group was longer than that in exhausted exercise group. The skeletal muscle expression of α-actin mRNA in model group was obviously higher than that in control group and in exhausted exercise group although the expression in electroacupuncture group was lower than that in model group, it’s higher than that in exhausted exercise group and in control group.Conclusion （1）The expression of α-actin mRNA in skeletal muscle could be increased after 3 weeks’ swimming train；（2）compared with