电针对大鼠脑缺血再灌注模型P_医学论文.docVIP

电针对大鼠脑缺血再灌注模型P_医学论文 电针对大鼠脑缺血再灌注模型P_医学论文 作者：刘玉珍，韩景献，蒋戈利，丁晓荣，陆明霞 【关键词】 针刺；,,脑缺血/再灌注；,,P 　　摘要：目的探讨针刺抗脑缺血再灌注炎性损伤的机理。方法采用线栓法制作大鼠大脑中动脉缺血模型（MCAO），采用免疫组织化学技术测定P-选择素和E-选择素表达的动态变化。结果在灌注3 h各实验组P选择素和E选择素均开始表达，二者均于再灌注24 h均达到高峰（组内比较P＜0.01），持续至再灌注48 h，针刺可显著降低P-选择素和E-选择素的表达（与模型组和非穴组比较，P＜0.01 ）。结论P选择素和E选择素的表达上调与再灌注后的炎症反应有关，早期针刺治疗可能通过下调脑缺血区黏附分子P选择素和E选择素的表达而防治脑缺血再灌注炎性损伤。 　　关键词：针刺； 脑缺血/再灌注； P选择素； E选择素 　　Effect of Galvano-acupuncture on the Expression of P-selectin and E-selectin of Cerebral Ischenic Region in Rats 　　Abstract：ObjectiveTo study mechanisms of early treatment of cerebral ischemia-reperfusion(I/R) by galvano-acupuncture in resisting inflammation injury in rats.MethodsCerebral I/R model was established by occlusion of the middle cerebral artery for 1h and then removal of the occlusion for 3 h ,12 h,24 h, and 48 h respectively .The expression of P-selectin and Eselectin was displayed by using imunohistochemical ABC in ischemic cerebral cortex and corpus striatum in rats.ResultsThe expression of P-selectin and Eselectin was detected at capillary walls in postischemic cerbral cortex at 1 h ischemia-3 h reperfusion and peaked at 1h ischemia-24 h reperfusion and increased continuously during 48 h reperfusion.The expression of both Pselectin and Eselectin in ischemic cerebral cortex were downregulated considerably by galvano-acupuncture treatment (P＜0.01) .ConclusionThe protective effect of acupuncture on cerebral neurons under acute infarction is possible through its downregulative action on the expression of Pselectin and Eselectin,which causes neutrophil infiltration that induced by adhesive molecules between endothelium cell and neutrophil. 　　Key words：Acuppuncture Cerebral ischemic/reperfusion Pselectin Eselectin 　　近年研究表明，脑缺血及脑缺血再灌注的病理过程中，周围血中性粒细胞向缺血区的迁移、浸润引发炎症反应加重了脑损伤，P选择素（Pselectin）和E-选择素（Eselectin）在白细胞与内皮细胞作用的起始阶段发挥重要作用。本课题拟建立大鼠局灶性脑缺血再灌注（ischemia/reperfusion,I/R）损伤模型，实施针刺干预，观察针刺对脑血区P选择素和E选择素表达的影响，旨在证明早期针刺治疗对脑缺血损伤的有效防治途径是通过对脑缺血后炎症反应的免疫调节机制实现的。 　　1 材料与方法 　　1.1 动物分组健康雄性Wis