电针调控大鼠胃酸分泌的机制_医学论文.docVIP

电针调控大鼠胃酸分泌的机制_医学论文 电针调控大鼠胃酸分泌的机制_医学论文 作者：赵保民，黄裕新，赵宁侠，刘梅，尚磊，黄梅，王庆莉 【关键词】 电针 　　关键词: 电针足三里胃酸胃肠激素 　　摘 要:目的 探讨电针足三里穴对大鼠胃酸分泌的调控机制. 方法 75只SD大鼠随机分为5组，在清醒状态下针刺双侧足三里穴，以空白对照、非经非穴作对照，三阴交穴作经穴对照.同步测定胃液量、pH值及胃液酸度，放免法测定血浆、胃液、胃粘膜胃泌素（Gas）和表皮生长因子（EGF）含量，透射电镜观察胃粘膜腺细胞超微结构. 结果 电针刺激足三里穴后大鼠空腹胃液量明显减少［（0.30±0.11）vs（0.63±0.12）mL，0.01］，胃液酸度明显降低［（30±3）vs（41±1）mmol L-1 ，0.05］，胃液pH升高.胃粘膜Gas含量增多［（136±17）vs（73±4）ng L-1 ，0.01］，血浆Gas含量下降［（84±22）vs（108±30）ng g-1 ，0.01］，胃液Gas无变化胃液及胃粘膜EGF含量显著升高［（3.2±1.1）vs（1.8±0.4）μg L-1 和（5.1±0.8）vs（2.4±0.4）μg g-1 ，0.01］，血浆EGF无变化.胃壁细胞泌酸小管不扩张，小管泡充盈不足，且数量不减少，非经非穴组及空白对照组各项指标无显著变化. 结论 电针足三里穴抑制大鼠胃G细胞Gas释放，降低血浆Gas，增加胃粘膜、胃液EGF含量电针足三里可改变胃壁细胞超微结构，抑制胃壁细胞泌酸小管功能、降低胃酸分泌. 　　Keywords:electroacupuncturezusanligastric acidgas-trointestinal hormones 　　Abstract:AIM To investigate the regulative mechanism of electroacupunture（EA）on gastric acid secretion in rats.METHODS SD rats（n=75）were randomized into5groups:Control group，non-point group，Sanyinjiao group，zasanli group，and unite group.All rats in consciousness were stimulated by electroacupuncture except control group.We measured the pH of fasting gastric juice as well as its vol-ume and acidity.Meanwhile radioimmunoassay was applied to assay the contents of gastrin（Gas）and epidermal grouth factor（EGF）in plasma，gastric juice and gastric mucosa tis-sue.Cellular ultra-microstructure of gastric mucosa coat was observed under a fransmission electron microscope.RESULTS After EA，it was discovered that the volume of the fasting gastric juice significantly reduced（0.30±0.11）vs（0.63±0.12）mL，0.01，and the acidity of the gastric juice significantly dropped（30±3）vs（41±1）mmol L-1 ，0.05，and the pH of gastric juice rose.EA stimulation of zusanli led to a drop of plasmic Gas（84±22）vs（108±30）ng L-1 ，0.01，a rise of Gas in gastric mucous coats（136±17）vs（73±4）ng g-1 ，0.01，but little change in Gas in gastric juice.Besides，there were some other changes ob-served.EGF of both gastric juice and gastric mucous coat in-creased（3.2±1.1）vs（1.8±0.4）μg g-1 ，0.