电针足三里对大鼠ir┐SP及细胞免疫的影响_医学论文.docVIP

电针足三里对大鼠ir┐SP及细胞免疫的影响_医学论文 电针足三里对大鼠ir┐SP及细胞免疫的影响_医学论文 作者：高巍，黄裕新，陈洪，赵宁侠，王庆莉，秦明，张洪新 【关键词】 针刺 　　关键词: 针刺足三里大鼠P物质免疫红细胞T细胞亚群 　　摘 要:目的 研究电针（EA）刺激大鼠足三里穴对免疫活性P物质（ir-SP）及细胞免疫的影响. 方法 将大鼠随机分成正常对照组、足三里组、免疫抑制模型组、非经非穴组、足三里+免疫抑制组.应用放射免疫法测定脑垂体和外周血ir-SP含量的变化应用流式细胞仪技术，通过微量全血直接免疫荧光染色法测定外周血T细胞亚群以反映细胞免疫功能应用红细胞C 3b 受体-酵母菌花环试验和红细胞-IC花环试验检测红细胞免疫功能. 结果 免疫抑制模型组大鼠脑垂体和外周血中ir-SP含量、外周血的CD4+ （0.01），RBC-C3b RR（0.05）明显低于正常对照组，CD8+ 无显著变化（Pgt0.05），足三里组+免疫抑制组各项指标与正常对照组相比无显著差异（Pgt0.05）.电针刺激足三里穴后正常大鼠脑垂体和外周血中ir-SP含量、外周血的CD4+ 、RBC-C3b RR、RBC-ICR均明显升高（0.01），CD8+ 无显著变化（Pgt0.05），且脑垂体和外周血中ir-SP含量与CD4+ 、CD4+ 和RBC-C3b RR呈显著正相关（r=0.744，0.05r=0.738，0.05r=0.719，0.05）.非经非穴组的各项指标与正常对照组相比无显著差异（Pgt0.05）. 结论 电针刺激足三里穴可提高正常大鼠及免疫抑制模型大鼠的细胞免疫功能和红细胞免疫粘附功能，同时，脑垂体和外周血中ir-SP的含量也明显升高，提示针刺治疗可以提高机体免疫力，其机制可能与ir-SP合成和释放增多有关，并通过该免疫递质对神经-内分泌-免疫调节网络发挥作用. 　　Keywords:acupunctureTsusanliratssubstance Pimmu-nityerythrocyteT-lymphocyte subgroups 　　Abstract:AIM To reveal the influence of electro-acupunc-ture（EA）both on the contents of substance P in centrum and peripheral blood and on its immune function in rats.METHODS The SD rats were divided into five groups，in-cluding control group，Zusanli group，immunosuppressive model group，none point group and immunosuppressive mod-el+EA group.The contents of ir-SP in centrum and periph-eral blood were determined by Radioimmunoassay.Also T lymphocyte subgroups of peripheral blood（including CD4+ and CD8+ ）were measured by direct microvolume whole blood immunoflurescent staining technique with flow cytometry（FCM）and erythrocyte immune function（including RBC-C3b RR and RBC-ICR）were observed to evaluate the immune functions of rats from different aspects.RESULTS ①The contents of ir-SP in centrum and peripheral blood，the value of CD4+ ，RBC-C3b RR and RBC-ICR in EA group were signif-icantly higher than those of control group（0.01），and the value of CD8+ was not statistically different between two groups.②The variation of CD4+ was significantly related with RBC-C3b RR（r=0.744，0.05r=0.738，0.05r=0.719，