硫化氢对甲醛炎性痛大鼠行为学的影响_医学论文.docVIP

硫化氢对甲醛炎性痛大鼠行为学的影响_医学论文 硫化氢对甲醛炎性痛大鼠行为学的影响_医学论文 作者：米琰 杨海扣 于水 季永 曾因明 【摘要】 目的观察鞘内注射硫化氢供体硫氢化钠(NaHS)和腹腔注射胱硫醚-γ-裂解酶抑制剂DL-炔丙基甘氨酸(PAG)对甲醛炎性痛大鼠的行为学影响，探讨小分子气体硫化氢(H2S)在炎性痛中的作用。方法成年雄性SD大鼠40只，随机分为5组(n=8)：疼痛模型组、人工脑脊液组、NaHS组、生理盐水组、PAG组。注射溶媒、NaHS或PAG后，向大鼠一侧足底皮下注射5%甲醛溶液，观察60 min，进行疼痛行为学评分。结果疼痛模型组、人工脑脊液组和生理盐水组的疼痛行为学评分差异无显著性(Pgt0.05)在第二相反应时段，与疼痛模型组相比，人工脑脊液组和生理盐水组的疼痛行为学评分无统计学差异(Pgt0.05)，NaHS组和PAG组的疼痛行为学评分明显降低(0.05)。 结论鞘内注射NaHS和腹腔注射PAG均能对甲醛炎性痛大鼠产生镇痛作用。 【关键词】 硫化氢硫氢化钠DL-炔丙基甘氨酸疼痛甲醛试验 　　Abstract: ObjectivesTo evaluate the effect of intrathecal pretreatment of sodium hydrosulfide (NaHS)-hydrogen sulfide donor and intraperitoneal injection of cystathionine-γ-liase inhibitor PAG on the ethology of rat with formalin-induced inflammatory pain, and to explore the role of hydrogen sulfide, a small-molecule gas in inflammatory pain. MethodsThere were no significant statistical differences in the scores of pain-induced ethology between the pain model group, ACSF group and the solvent group (Pgt0.05). In the second phase of response, there were no significant statistical differences in the scores of pain-induced ethology in ACSF group and the normal saline group, as compared to the pain model group (Pgt0.05), while those in NaHS group and PAG group showed a significant decrease (0.05). ResultsControl group and solvent group of pain behavioral score had no significant statistical difference(Pgt0.05). In the second period, compared to control group, rat pain behavior scores in ACSF group and saline group had no significant statistical difference(Pgt0.05),but that of NaHS group and PAG group reduced significantly(0.05). ConclusionBoth intrathecal injection of NaHS and intraperitoneal injection of PAG could produce anti-nociceptive effects. 　　Key words: H2S NaHS PAG pain formalin test 　　H2S作为继NO、CO后的第3种内源性气体信号分子，是近20年来才开始对其生理、病理方面的研究和认识。长久以来，对于H2S的研究主要集中于它的毒性作用，如中枢神经系统和呼吸系统的影响。H2S具有与NO、CO类似的特性：小分子量气体，不通过受体发挥作用，可以自由通过细胞膜，其生成受内源性关键酶的调节，生理浓度时有很明确的特殊功能等。H2S可内源性产生，可存在于生物体内的多个系统中，并通过多种调节方式和信号转导形式发挥生理病理作用。近