TRAIL、MAP2和Nestin在流产患者绒毛组织中的表达及意义.docVIP

TRAIL、MAP-2和Nestin在流产患者绒毛组织中的表达及意义 朱旅聪1 刘学红2 张泳2 * （1绍兴文理学院附属医院，2绍兴文理学院医学院 浙江 绍兴 312000 ） [摘要] 目的 探讨TRAIL、MAP-2和Nestin与自然流产和正常人流绒毛发育的关系。方法 应用免疫组织化学法检测第40-90天自然流产和正常人流患者胎盘蜕膜和绒毛组织中TRAIL、MAP-2和Nestin的表达规律。结果 第40-90天的自然流产患者的绒毛和蜕膜组织内TRAIL蛋白阳性表达数明显高于正常人流，Nestin在绒毛组织中的阳性表达则相反。MAP-2在正常人流绒毛组织中呈阴性表达，在自然流产患者的绒毛组织中呈阳性广泛表达。结论TRAIL、MAP-2和Nestin的表达状况与绒毛组织细胞发育及妊娠关系密切。 [关键词] 自然流产；绒毛；巢蛋白；微管相关蛋白2；TRAIL；免疫组织化学 Expressions of TRAIL, MAP-2 and Nestin and its significance in the villus tissue of the normal human and spontaneous abortion ZHU Lvchong, LIU Xuehong，ZHANG Yong * (1 Affiliated hospital, 2 Medical College, Shaoxing University, Shaoxing City, 312000, China) [Abstract] Objective To investigate the relationship between TNF-related apoptosis inducing ligand (TRAIL), Microtubules related protein 2(MAP-2), Nestin and villus development of the normal human and spontaneous abortion . Methods The expressive rule of TRAIL, MAP-2, Nestin in the decidual and villus tissues of the spontaneous and normal abortion was observed by immunohistochemistry between the 40th and the 90th day. Results The positive expression number of TRAIL protein in the chorionic villus was obviously higher than that of normal abortion, but Nestin positive expression in villi tissue of the opposite. MAP-2 was negative expression in the normal villi tissue, and positive widely expression in patients with spontaneous abortion villus tissues. Conclusion The expressional situation of TRAIL, MAP-2, Nestin in the decidual and villus tissues is closely related to the villi tissue cell development and pregnancy. [Key words] Spontaneous abortion; Villus; Nestin; Microtubules related protein 2(MAP-2); TNF-related apoptosis inducing ligand (TRAIL)；Immunohistochemistry 【收稿日期】 【修回日期】 【基金项目】浙江省教育厅项目（Y201328640）绍兴市哲学科学规划项目（125071） *通讯作者，张泳，安徽歙县，实验师. E-mail: E-mail: hhren6588@ 电话：0575自然流产是常见的妊娠并发症，在育龄妇女中的发病率高，其中不明原因自然流产约占自然流产发生率的50%。胎盘是胎儿与母体接触面，绒毛的正常发育是胚胎正常发育的前提和关键。肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptos