百草枯对木质素降解产酶及其生物化学变化的影响.docVIP

百草枯对木质素降解菌产酶及其生物化学变化的影响 赵芸晨1,2, 李建龙1, 陈育如3, 黄海霞1, 余醉1 1 南京大学生命科学院 国家医药重点实验室, 南京 2100932 河西学院农学系, 张掖 7340003 南京师范大学生命科学学院, 南京 210046 摘 要: 为研究外源活性氧对木质素降解菌的影响, 本实验对外源百草枯诱导下的杂色云芝(Coriolus versicolor)产酶及其生物化学过程进行了研究。将一定浓度的百草枯加入培养7 d的杂色云芝菌培养液中, 连续培养148 h, 测定其胞外木质素降解酶、胞内抗氧化酶的活性及生物化学参数的变化。与对照相比, 30 ?mol/L的百草枯能够显著促进杂色云芝锰依赖过氧化物酶(MnP)、木质素过氧化物酶(LiP)和漆酶(Lac)的活性, 3种酶活性分别提高了1.3、7和2.5倍; 在连续培养的前48 h, 30 ?mol/L的百草枯促进了胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性。百草枯对于胞外木质素降解酶活性的促进作用比对胞内抗氧化酶活性的促进作用明显。百草枯的加入促进了胞外酚类化合物与甲醛的浓度的增加, 而丙二醛的浓度在培养的前24 h内增加, 随后下降。结果表明, 百草枯的加入对白腐菌产生了氧化胁迫, 但菌株的抗氧化系统能够有效地进行氧化剂的清除, 从而阻止氧化剂对机体的氧化伤害。百草枯作为外源氧化胁迫剂, 可以增加木质素降解酶活性, 有利于木质素的降解。 关键词: 杂色云芝, 百草枯, 氧化胁迫, 木质素降解酶, 甲醛 Impact of exogenous paraquat on enzyme exudation andbiochemical changes of lignin degradation fungi Yunchen Zhao1,2, Jianlong Li1, Yuru Chen3, Haixia Huang1, and Zui Yu1 1 State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China 2 Department of Agronomy, Hexi University, Zhangye 734000, China 3 School of Life Science, Nanjing Normal University, Nanjing 210046, China Abstract: To study the effect of exogenous oxygen, we added water solution of paraquat to 7 d cultures of Coriolus versicolor for the next 148 h. Enzyme exudation and biochemiscal process were investigated on the addition of paraquat. We found that compared with the control (without paraquat), the addition of 30 ?mol/L paraquat stimulated the activity of manganese dependent peroxidase (MnP), lignin peroxidase(LiP), and laccases (Lac) 7, 2.5 and 1.3 times, respectively. Also, addition of paraquat enhanced activity of superoxide dismutase (SOD) and catalase (CAT) in the first 48 h. Impact of paraquat on ligninolytic enzymes was significant than that on antioxidant enzyme. Addition of paraquat enhanced phenolic compounds and formaldehyde of cultures too. And concentration of malondialdehyde was increased in the first 24 h. The result