花生壳中黄酮类化合物的提取及其纯化.pdfVIP

※工艺技术 食品科学 2009, Vol. 30, No. 08 101 花生壳中黄酮类化合物的提取及其纯化 李芳清，徐卫东 (东华理工大学应用化学系，江西抚州 344000) 摘 要：本实验采用微波法对花生壳中黄酮类化合物进行提取。将单因素分析与正交试验相结合，得到微波法提 取花生壳中黄酮类化合物的最佳工艺条件：提取剂乙醇体积分数50 % ，微波功率700W ，提取时间4min ，料液比 1:30(g/ml) ，每克花生壳中提取黄酮类化合物为0.48mg ，回收率为93.3 % 。微波法与乙醇回流法相比提取时间由4h 减少到4m in ，提取黄酮类化合物的量提高了2 .4 倍。大孔吸附树脂富集纯化花生壳黄酮类化合物方法：以木犀草 素作为考察指标，考察静态吸附量和洗脱率，筛选出最适型号树脂，比较纯化前后黄酮类化合物和木犀草素含量 的变化。结果表明：D 10 1 型树脂为花生壳提取液最佳精制纯化树脂，树脂纯化后黄酮类化合物和木犀草素的含量 提高4 ～5 倍。 关键词：花生壳；微波法；黄酮；吸附树脂；木犀草素 Extraction and Purification of Total Flavonoids from Peanut Hulls LI Fang-qing ，XU Wei-dong (Department of Applied Chemistry, East China Institute of Technology , Fuzhou 344000, China) Abstract ：Based on single-factor test and orthogonal test, microwave-assisted extraction condition s of total flavonoid from peanut hulls were optimized. The results showed that the optimal extraction conditions are as follows: taking 50% alcohol as extraction solvent, microwave power 700 W, radiation time 4 min and material to extraction solvent ratio 1:30 (g/ml). Under the above conditions, the yield and spike recovery of total flavonoid are 0.48 mg/g and 93.3%, respectively. Compared to ethanol reflux extraction, microwave-assisted extraction not only can reduce the extraction time from 4 h to 4 min but improve the yield by 2 .4 times. Furthermore, the purification of total flavonoids from peanut hulls by macroporou s adsorption resin was also investigated in the present study. Using the luteolin content as the index, the capabilities of static adsorption and desorption were examined. The results showed that the D101 resin is the best among chosen 7 resins for the purification, and both of contents o