SARS-CoVN蛋白抗原表位的筛选和鉴定.pdfVIP

第 21 卷 4 期 中 国 病 毒 学 21 （4 ）：314-318 2006 年 7 月 VIROLOGICA SINICA July 2006 SARS-CoV N 蛋白抗原表位的筛选和鉴定* 1 1 2 1 1 1 1** 黄 欣 ，高亚萍 ，王希良 ，辛忠涛 ，董 洁 ，邵宁生 ，柳 川 （1.军事医学科学院基础医学研究所，北京 100850；2.军事医学科学院微生物流行病研究所，北京 100071） Screening and Identification of the SARS-CoV N Protein Epitopes 1 1 2 1 HUANG Xin ，GAO Ya-ping ，WANG Xi-liang ，XIN Zhong-tao ， 1 1 1** DONG Jie ，SHAO Ning-sheng ，LIU Chuan (1.Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 2. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China) Abstract ：The epitopes of SARS-CoV were screened from a 12-mer phage display random peptide library using anti- SARS-CoV horse polyclonal antibodies of as a target. The phage clones enriched in biopannings were sequenced. Two consensus sequences were obtained, DXXDP and TXTLL, which had close identity to the 341-345 aa and 392-396 aa of SARA-CoV N protein sequences,respectively. The phage clones with consensus sequences and the N protein were both recognized and bound by the antibodies in a competitive-inhibition ELISA test. The consensus sequence peptides were cloned and displayed on the bacterial flagellin. Balb/c mice were vaccinated using the reconstruted bacteria and the collected serum was shown to speccifically combined with SARS-CoV N protein. This c