细菌Flavobacteriaceae sp.CZ1127产海参岩藻聚糖硫酸酯酶的发酵条件优化.pdfVIP

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.3 细菌Flavobacteriaceae sp.CZ1127产海参岩藻聚糖 硫酸酯酶的发酵条件优化 王春霞，段高飞，唐庆娟，常耀光，薛长湖 （中国海洋大学，山东青岛 266003） 摘要：以从海洋中分离筛选得到的细菌Flavobacteriaceae sp. CZ1127为研究对象，通过单因素实验和正交试验对其产海参岩藻聚 糖硫酸酯酶的培养基成分进行优化，提高其产酶量。得出其最佳培养基组成为(w/v)：海参岩藻聚糖硫酸酯0.25% 、酵母粉0.15%、 MgSO ·7H O 0.05%、CaCl 0.02%、KCl 0.01%、Na HPO 0.22%、NaH PO 0.06% 、NaCl 2.5%；确定了该菌株的最佳培养条件为：500 4 2 2 2 4 2 4 mL的三角瓶装液150 mL、温度为25 ℃、转速150 r/min、发酵时间为48 h。优化后，海参岩藻聚糖硫酸酯酶活力为22.88 U/mL ，比优 化前提高了3.01倍。 关键词：海参；海参岩藻聚糖硫酸酯；海参岩藻聚糖硫酸酯酶；发酵条件 文章篇号：1673-9078(2011)3-332-336 Optimization of the Growth Conditions for a Focoidanase-producing Marine Bacterium Flavobacteriaceae sp. CZ1127 WANG Chun-xia, DUAN Gao-fei, TANG Qing-juan, CHANG Yao-guang, XUE Chang-hu (Ocean university of China, Qingdao 266003, China) Abstract: Flavobacteriaceae sp. CZ1127, a fucoidanase-producing strain, was isolated and the optimal conditions for fucoidanase fermentation by Flavobacteriaceae sp. CZ1127 were optimized by single-factor experiments and orthogonal experiments. The optimum culture medium was obtained as: sea cucumber fucoidan 0.25%, yeast 0.15%, MgSO ·7H O 0.05%, CaCl 0.02%, KCl 0.01, Na HPO 0.22%, 4 2 2 2 4 NaH PO 0.06%, NaCl 2.5%. After cultivated in 150mL medium with 500mL shake flask at 25 ℃for 48 h with shaking speed of 150 r/min, the 2 4 activity reached the highest value of 22.88 U/mL which was 3.01 times higher than that under the original conditions. Key words: sea cucumber; sea cucumber fucoidan; fucoidanase; fermentation