不同蚕蛹油中脂肪酸的性状及组分分析.pdfVIP

148 2011, Vol. 32, No. 04 食品科学 ※分析检测 不同蚕蛹油中脂肪酸的性状及组分分析 1 1 1 ,2 1 1 , 潘文娟 ，方婷婷 ，廖爱美 ，张海祥 ，魏兆军 * ( 1.合肥工业大学生物与食品工程学院，安徽合肥 230009 ；2 .合肥师范学院生命科学系，安徽合肥 23006 1) 摘 要：研究不同蚕蛹油的脂肪酸种类和相对含量。采用气相色谱- 质谱方法，对KOH/CH3 OH 酯交换法甲酯化4 种不同的蚕蛹油进行定量分析。结果表明：柞蚕蛹、蓖麻蚕蛹、家蚕蛹及剿丝后家蚕蛹油的酸价、皂化值和过 氧化值存在显著差别；4 种蛹油的脂肪酸成分的种类都以棕榈酸、硬脂酸、油酸、亚油酸、α- 亚麻酸为主，但 含量存在显著区别；4 种蚕蛹油中，柞蚕蛹油的不饱和脂肪酸相对含量是最高的，达7 7 .2 9 % ；蓖麻蚕蛹油的α- 亚麻酸相对含量最高，为50 .52 % ；缫丝后家蚕蛹油不饱和脂肪酸的相对含量比缫丝前降低。 关键词：蚕蛹油；脂肪酸组分；气相色谱- 质谱 Character and Fatty Acid Composition of Silkworm Pupal Oil 1 1 1,2 1 1, PAN Wen -j uan ，FANG Tin g-ting ，LIAO Ai-mei ，ZHANG Hai-xiang ，WEI Zhao-j un * (1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China ； 2. Department of Life Science, Hefei Normal University, Hefei 23006 1, China) Abstract ：In order to determine the types an d relativ e content s of fatty acids in 4 k ind s of silkworm pup al oil, oil transesterification was carried out with KOH/CH3OH, followed by gas chromatography-mass spectrometric analysis. Results indicated that acid values, saponification values and peroxidation values differed significantly among the 4 kinds of pupal oil. All the four tested materials contained the following maj or components: linolenic acid (LNA), palmitic acid, stearic acid, oleic acid and α-linolenic acid, which exhibited different relative contents. Among them, the relative content of unsaturated fatty acids in oak silkworm pupal oil was the highest, which was up to 77.29% and castor silkworm pupal oil contained the most α-linolenic acid, with a relative content of 50.5