黑果枸杞叶片中多糖提取工艺研究.pdfVIP

黑果枸杞叶片中多糖提取工艺研究 吴翠云匕汪河滨鼍李万福2，白红进埘 (1．塔里木大学塔里木盆地生物资源保护利用兵团重点实验室，新疆阿拉尔843300；2．塔里木大学植物科技学 院，新疆阿拉尔843300；3．塔里木大学生命科学学院，新疆阿拉尔843300) 摘要：采用水浴法、微波辅助提取法与超声一微波协同萃取法提取黑果枸杞叶片多糖，确定最适提取方法。通过单因素 试验和正交试验确定最优提取工艺。采用蒽酮一硫酸法测定多糖含量。结果表明：最佳提取方法为超声一微波协同萃取 法。最优提取条件是：料液比为1：25，提取温度为90℃，提取时间为30min，多糖平均提取率为lO．07％，RSD=1．60％ (n_3)。回收率loo．7％，RsD=1．84％(n=3)。 关键词：黑果枸杞叶片；多糖；提取工艺 STUDY0NTHE Of’POLYSACCHARⅡ)E EXTRAC’110NTECHNOLOGY WU He-binl一，UWan—fu2，BAI Cui—yunl一，WANG Hong—jinl，3t‘ of in of ProtectionUtilizationof ResourcesTarimBasin Productionand (1．KeyLabomtory Bi0109ical Xinjiang Construction ofPlamScienceand Technology，T秭m Corps，TarimUniversity，Alar843300，Xinjiang，China；2．Colkge of“fe Science，T撕m UniversityAlar843300，xinjiarIg，China；3．CoUegeUniVersity’mar assistedandultrasonic—microwaveassistedextraction were Abstract：Water-bat王lmicmwave techniquesapplied to 1eavesandt}le extmctionme出odw鹬selected extract f而m￡”i啪彤舭n如Ⅱm polysaccharides optimal by and witllothers．ExtractionConditionswereassureda factor compared bysin91e orthogonalexperiments．Content showed of wasde￡emined acid results thatUltfasonic— polysaccharide8 byaIlthrone—sulphudcco王orimetry．The micmwaveextractionw鹊the extI-actionmethod．Theresults indicatedthat