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INSTRUCTIONS NE-PER® Nuclear and Cytoplasmic Extraction Reagents 78833 78835 0872.5 Number Description 78833 NE-PER Nuclear and Cytoplasmic Extraction Reagents, sufficient reagents for extracting 50 cell pellet fractions having packed cell volumes of 20µL each (a total of ~2g cell paste) Kit Contents: Cytoplasmic Extraction Reagent I (CER I), 10mL Cytoplasmic Extraction Reagent II (CER II), 550µL Nuclear Extraction Reagent (NER), 5mL 78835 NE-PER Nuclear and Cytoplasmic Extraction Reagents, sufficient reagents for extracting 250 cell pellet fractions having packed cell volumes of 20µL each (a total of ~10g cell paste) Kit Contents: Cytoplasmic Extraction Reagent I (CER I), 50mL Cytoplasmic Extraction Reagent II (CER II), 2.75mL Nuclear Extraction Reagent (NER), 25mL Storage: Upon receipt store kit components at 4°C. Product is shipped at ambient temperature. Introduction The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagents enable stepwise separation and preparation of cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. Non-denatured, active proteins are purified in less than two hours. Addition of the first two reagents to a cell pellet causes cell membrane disruption and release of cytoplasmic contents. After recovering the intact nuclei from the cytoplasmic extract by centrifugation, the nuclei are lysed with a third reagent to yield the nuclear extract. Extracts obtained with this product generally have less than 10% contamination between nuclear and cytoplasmic fractions