佛波酯对人胃癌细胞Ha_ras基因启动子及启动子结合蛋白的影响.pdfVIP

佛波酯对人胃癌细胞Ha_ras基因启动子及启动子结合蛋白的影响.pdf

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佛波酯对人胃癌细胞Ha_ras基因启动子及启动子结合蛋白的影响.pdf

37 1 Vol37,No1 20062 ACTA ANATOMICA SINICA Feb. 2006 Haras 高萍 王永军 柳惠图* ( , 100875) [] (PMA)B C823 Haras RTPCR prasEYFP , Southwestern blotting PMA( 100gL) B C823 72 96h , Haras Haras Haras PMA( 100gL) 72 96h, ,B C823 Haras ,Haras 652%718%; Haras ,, 18kD, 35kD PMA Haras Haras ,Haras [] ;Haras ;Haras ; B C823; ; DNA [] Q253 [ ] A [ ] 2006)0162 EFFECT OF PMA ON THE ACTIVITY OF Haras PROMOTER AND PROM OTER BINDING PROTEIN IN HUMAN GASTRIC CARCINOMA CELLS * AO Ping, WAN Yongjun, LIU Hui tu (Key Laboratoryf or Cellprolfieration and Regulation of Ministry of Education, Insititute of Cell Biology, College of Lfie Science, Bejiing Normal niversity, Bejiing 100875, China) [Abstract] Objective To study the effect of PMA on the activity of Haras promoter and promoterbinding protein in B C823 cells. Meth ds Human gastric carcinoma cells( B C823) were treated with PMA( 100 gL) for 72 hours and 96 hours respectively. By using tripure isolation reagent the RNA of treated and untreated cells were extracted according to the procedure of the manufacturer. The expression of Haras gene was detected by reversed transcriptase polymerase chain reaction. The recombinant plasmid pRasEYFP which contained the regulation fragment of Haras and EYFP gene was constructed. Haras regulation fragment was located upstream EYFP gene. The recombinant plasmid pRasEYFP wastransiently transfected to B C823 cells. Using the EYFP as reporter, the activity

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