个体化医疗的现状与未来蛋白质组学.pptVIP

个体化医疗的现状与未来三. 蛋白质组学研究 吕林莉 M.D., Ph.D. 东南大学医学院 Outline Why we study proteome? What is proteome? How to study proteome? Proteomics and disease Why we study proteome? Why we study proteome?为何要研究蛋白质组？ Gene expression not correlates with protein All proteins have life and death cycle Structure and chemistry property of protein is more complex (1) Gene expression not correlates with protein The abundance of cellular protein is affected by: (a) DNA transcription rate (转录效率）(codon bias)（密码子偏倚） (b) mRNA translation rate（翻译效率） (c) Protein degradation rate（降解效率） Apparent poor relationship betweengene expression and protein content (2) The life and death of a protein-dynamic change (3) Structure and chemistry property of protein is more complex 细胞内基因水平并不直接反映蛋白质水平 蛋白质是机体功能的真正执行者 蛋白质的数目、结构和理化性质都更为复杂。 What is proteome? What is Proteome? Proteome - All the proteins expressed by a cell , tissue or organism at a particular time and under specific conditions. Branches of Proteomics Expressional Proteomics Functional Proteomics Structure Proteomics Structural Proteomics How to study proteome? The Major Techniques in Current Proteomics 2D Gel-Electrophoresis Protein separation Molecular weight (MW) Isoelectric point (pI) Staining Birds-eye view of protein abundance 质谱分析 第一台质谱仪是英国科学家弗朗西斯·阿斯顿于1919年制成的。 阿斯顿用这台装置发现了多种元素同位素，研究了53个非放射性元素，发现了天然存在的287种核素中的212种，第一次证明原子质量亏损。 他为此荣获1922年诺贝尔化学奖。 Mass Spectrometer(质谱分析仪）三个基本组成部分 Peptide Mass Fingerprint Peptide Mass Fingerprint Peptide Mass Fingerprint Proteomics and disease Collapse of the single target paradigm-the need for systems biology While genomics can assess a patient’s risk for certain diseases, proteomics can tell you much more about what’s actually going on in the patient’s body. 蛋白质组学技术的应用 “Proteomics is the heart of personalized medicine—more so than genetics, When you’re born, you’re healthy. And when you’re 80, you’re dying, but you’ve got more or less the same genome as when you were healthy.” Diseas