Amplification and cloning of infectious bursal disease v.pptVIP

Amplification and cloning of infectious bursal disease virus genomic RNA segments by long and accurate PCR Aydemir Akin, Ching Ching Wu*, Tsang long Lin Abstract RNA denaturation by heating RTase lacking RNase-H activity RNase-H digestion Long and accurate PCR(LA-PCR) Utility for any IBDV strains or isolates Table of contents 1. Introdction 2. Material and methods 2.1. Virus 2.2. Extraction of viral RNA 2.3 Oligodeoxynuclotide primers 2.4. Reverse transcription and LA-PCR 2.5. Cloning of cDNA amplicons of small and large IBDV genome segments 2.6 nucleotide sequencing 3. Results and discussion