cDNA microarrays.pptVIP

Panu Somervuo, March 19, 2007 cDNA microarrays cDNA microarrays small slides with several measurement units, spots e.g. 2.5cm-by-7.6cm glass slide with 30,000 spots each spot contains specific nucleotide sequences, probes in hybridization process, labeled (Cy5, Cy3) samples attach to probes comparative genome hybridization (CGH): DNA samples gene expression: RNA samples relative intensity of hybridization can be measured Data flow biological data, DNA/RNA extraction, fluoresence dye labeling, hybridization?array scanning?image image processing: spot segmentation?datafile data preprocessing