Human Genome Project.pptVIP

Human Genome Project Basic Strategy How to determine the sequence of the roughly 3 billion base pairs of the human genome. Started in 1995. Various side projects: genetic diseases, variations between individuals, ethnic variation, comparison to other species. Strategy: 1. physical map relating specific DNA markers to the proper chromosomal position. 2. Overlapping set of cloned DNAs (contigs) 3. sequencing and assembly 4. finding the genes in the sequence 5. annotation of gene function Physical Maps A genetic map uses recombination, crossing over during meiosis, to determine how frequently two