AFFYMETRIX chips.pptVIP

AFFYMETRIX chips What are DNA chips/arrays? Small silica (glass) chips covered with thousands of nucleotides of know sequence Underlying principle: Base pairing or hybridization Medium for matching known and unknown DNA samples Two types of arrays-Macro and Micro Macro-Sample spot sizes = 300 microns Micro-Sample spot sizes 200 microns in diameter Micro Arrays Fabricated by high-speed robotics; generally on glass, but sometimes on nylon substrates Known probes used to determine complementary binding Enable massive parallel gene expression and gene studies Dramatic increase in thro