A Study of Starch Metabolizing Proteins Pam Brewer-Micha.pptVIP

A Study of Starch Metabolizing Proteins Pam Brewer-Michael1 , Tracie Hennen-Bierwagen2, Myers /James Laboratory2 1Marshalltown Community School District, 2Iowa State University, Ames, Iowa * ABSTRACT In order to perform in vitro protein-protein interaction studies of enzymatic Maize proteins in relation to starch architecture, working quantities of the proteins of interest must be available for study. Five Maize proteins were affinity purified from E.coli cultures transformed with pET and Gateway vectors containing N-Terminal affinity tags fused to cDNAs coding full length or specific dom