全反式维甲酸对人卵巢癌细胞糖原代谢影响的实验研究.pdfVIP

2005 3 30 3 Med J Chin PLA Vol 30 No 3 March 2005 237 全反式维甲酸对人卵巢癌细胞糖原代谢影响的实验研究 刘 琦 刘春敏 姜少军 吴 波 孟 奎 刘小转 施正良 石群立 ( ) ( ) COC2 , , ; 2mg/ (kg d) 4 CAOV3 , COC2 5mol/ L 10mol/ L , 30mol/ L ; CAOV3 , COC2 CAOV3 , , , ; ; ; R73731 Experimental study on the change in intracellular glycogen in ovarian cancer after alltrans retinoic acid treatment Liu Qi, Liu Chunmin, Jiang haojun et al . Department of Obsterics and Gynecology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China Abstract Objective To investigate the effect of alltrans retinoic acid on the change in intracellular glycogen in ovarian epithelioma cell line in v itro and ovarian epithelial carcinoma in nude mice in v iv o. Methods COC2 cells were treated with alltrans retinoic acid in 1, 5, 10 and 30mol/ L drug concentrations for different length of time, and then intracellular glycogen and LDH were determined by bio chemistry assay. Morphologic changes were observed with light and electron microscopy. CAOV3 tumorbearing nude mice were treated with intragastric injection of the same drug in a dose of 2mg/ (kg d) for four weeks. The tumor samples were harvested thereafter for pathological study with histochemical and immunohistochemical staining, and also with electron microscopy. Results Intracellular glycogen was significantly increased, while LDH level was lowered after the cell line was treated with 5~ 10mol/ L of alltrans retinoic acid, and apoptosis of cancer cell occurred after using 30mol/ L of the drug. These changes were also observed in CAOV3 cells of tumorbearing nude mice. Conclusion Our results suggest that treatment with alltrans retinoic acid resultin an increase in intracellular glycogen and de crease in LDH level both in COC2 cells in v itro and in CAOV3tumorbearing nude mice in v iv o, and the suppression of tumor cell prolifer ation may be attributed to retarded intracellul