Protein Expression in E. coli using pEX Expression Vecto.pptVIP

Protein Expression in E. coli using pEX Expression Vectors Validation Data 5 New E. coli Expression Vectors Please see vector maps at /cdna/TrueORF/DestinationVector.aspx then click on “Bacterial expression” tab Protein Expression in E. coli N-tagged vector 4 different mammalian ORFs was cloned into the pEX-N-His-GST vector. GST-fused proteins were expressed at high levels with all constructs. 1 2 pEX-C-His-Bin1 (NM_139351) no IPTG pEX-C-His-Bin1 (NM_139351) with IPTG BIN1 Protein Expression in pEX-C-His Vector 191 97 64 51 39 28 MW (kDa) The ORF of Bin1 was cloned into pEX-C-His