Reverse Genetics of RNA Viruses.pptVIP

Reverse Genetics of RNA Viruses Chang Won Lee, DVM, Ph.D. Lee.2854@osu.edu Phone: 330-263-3750 Reverse Genetics (RG) The creation of a virus with a full-length copy of the viral genome The most powerful tool in modern virology RG of RNA viruses Generation or recovery (rescue) of infectious virus from cloned cDNA Nature of RNA viruses Polarity (+ sense or – sense) Size of the genome Segmented or not Site of replication (nucleus or cytoplasm) Families of RNA Viruses Polarity Plus-stranded RNA viruses - deproteinated genomes of these viruses are able to utilize the host cell machinery to ini