胃粘膜表面局部投予乙酸制备大鼠胃溃疡模型的方法.pdfVIP

解剖科学进展　Progress of Anatomical Sciences　2011 Jan,17(1):8~11,15 胃粘膜表面局部投予乙酸制备大鼠胃溃疡模型的方法 才丽平，蒋　宁，曲　怡，林庶茹，韩荣春，王　浩，郑洪新* （辽宁中医药大学 基础医学院中医分子生物学实验室 ，辽宁 沈阳 110032） 【摘要 】　 目的　对大 鼠胃溃疡模型制备方法进行改进 。方法 胃粘膜表面局部投予乙酸制备大 鼠胃溃疡模 型。10%水合氯醛（3.5ml/kg体重）肌肉注射麻醉，用0.5％碘伏消毒大 鼠腹部皮肤，沿左肋下缘开腹，将胃轻轻 拉 出。用带有 圆环 的 胃溃疡制备镊子夹住距 幽 门部3mm胃体部 区域 。在夹 闭区的 胃腔 内注入0.18ml矿物油和 0.02ml 60%乙酸，45sec后从 胃部吸出乙酸，再 向胃腔内注入2ml生理盐水，1min后 吸出。将胃复位，逐层关腹 。 结果 该方法可在大 鼠胃部造成单个、 圆形、大小、深度一致 的溃疡灶，并且解决了手术引起粘连 的问题。结论 本文提供一种改进 的胃粘膜表面局部投与乙酸制备大 鼠胃溃疡模型的方法，该模型可用于考察消化性溃疡 的愈 合过程、抗溃疡药物疗效评价、药物对胃粘膜所产生的副作用监测等。 【关键词】大鼠；胃溃疡；模型 + 【中图分类号】　R656.6 2 【文献标识码】　A 【文章编号】　1006-2947（2011）01-0008-04 Establishment of rat gastric ulcer model by applying acetic acid to specified area in rats gastric mucosa CAI Li-ping, JIANG Ning, QU Yi, LIN Shu-ru, HAN Rong-chun, WANG Hao, ZHENG Hong-xin* （Molecular Biology Lab, School of Preclinical Medicine of Liaoning University of TCM, Shenyang 110032 China） 【 Abstract】 　 Objective　 To improve the established method of acetic acid-induced rat gastric ulcer model. Methods The rat gastric ulcer model by applying acetic acid to specified area in rats gastric mucosa. After 10% chloral hydrate intramuscular anesthesia (3.5ml/kg), the rats skin of abdomen was sterilized by 0.5％ iodophors. The rats abdomen was opened along left subcostal margin and the stomach was gently pulled out. The body of stomach approximately 3 mm proximal to the pylorus was clamped by round forceps. Then a mixture of mineral oil(0.18 ml) as well as 60% acetic acid(0.02 ml) were injected into the clamped lumen, which was removed after 45 seconeds. After that, normal saline(2ml) was injected into the gastral cavity, which was removed after