LC-MS法测定人血浆中氯雷他定浓度.pdfVIP

LC—MS法测定人血浆中氯雷他定浓度 附属第二医院，广州市510120)一 中图分类号 R969．1 文献标识码 A 文章编号 1001—0408(2008)17—1314—03 摘要 目的：建立以液一质联用法测定人血浆中氯雷他定的方法。方法：血浆样品中加入内标地西泮，用液相萃取法处理后测定， 色谱柱为AgilentTC—C18，流动相为乙腈(含1％甲酸)一0．02m01．L。甲酸铵水溶液(90：lO)，流速为0．8mL·rain～，柱温为 383．2-*337．0 40℃。通过电喷雾电离源(ESI)，质谱在正离子多反应监测模式(MRM)下行特征母一子离子对信号采集，以m／z (氯雷他定)和m／z285．1—154．0(地西泮)进行定量分析。结果：氯雷他定检测浓度在0．5～1001范围内线性关系良好 btg·L (r=0．996 便、灵敏，适用于人体内氯雷他定血药浓度测定及临床药动学研究。 关键词液一质联用法；氯雷他定；血药浓度 DeterminationofLoratadineinHumanPlasmaLC。。MS by QIUChang，LI Zhen—huan，CHEN Xiao—fang，WENYu—guan，ZHAO Wei-jia(GuangzhouPsychiatricHospi— tal，Guangzhou510370，China) LI SecondAffiliated of 510120，China) Jian—fang(The HospitalZhongshanUniversity，Guangzhou ABSTRACT0BJECTIVE：ToestablishanLC—MSmethodforthedeterminationofloratadineinhuman plasma．METH 0DS：The wastreated extraction。withastheinternalstandard．Theof plasmasample byliquidphase diazepam separation was onan columnwithacolumn of40℃．Themobile consistedoface- sampleperfo瑚edAglientTC—C18 temperature phase 1 1％methanoicacid)一0．02mol·LammoniumformateSOl(90：10)ataflowrateof0．8mL·min-1． tonitrile(including aq LoratadinewasdetectedbvLC—MSunder ionizationMRMmode．The was positiveelectrospray quantificationperformed basedonthe of ionm／z peakintensity 383．}+337．0(10ratadine)andm／z fragment 1 Thelinear curvewasobtainedinthe of for lower