尖孢镰刀菌寄主专化型脂肪酸生物标记鉴别特性的判别模型的.docVIP

尖孢镰刀菌寄主研究( 刘波*，肖荣凤（福建省农业科学院农业生物资源研究所，福建 福州 350003，，） 摘要：分析不同寄主尖孢镰刀菌脂肪酸分布特性Bayes逐步判别从供试的2个菌株中检测到8种脂肪酸，其中60%以上主要脂肪酸16:0、18:0 和18:2 CIS 9,12/18:0a 。聚类分析，与菌株的来源对应关系。脂肪酸主成分分析得到尖孢镰刀菌脂肪酸中的特征脂肪酸有5个，分别为14:0，16:0，16:1 CIS 9 (W 7)，18:0和18:2 CIS 9,12/18:0aBayes逐步判别分析Y1=-183.06+12.24X1+ 8.05X2+5.39X4+3.58X5，Y2=-174.09+10.40X1+7.96X2+5.40X4+3.42X5，Y3=-173.97+14.85X1+7.25X2+6.58X4+3.53X5，当1Yi﹤2时，的寄主为西瓜； 当2Yi﹤3时,的寄主为香蕉；当 Yi﹥3时，的寄主为花生判对的概率0.90909，【结论】脂肪酸生物标记可以作为尖孢镰刀菌寄主鉴别指标，脂肪酸14:0， 16:0， 18:0， 18:2 CIS 9,12/18:0a是判别尖孢镰刀菌寄主的四个重要指标关键词：尖孢镰刀菌；脂肪酸 for forma specials of Fusarium oxysporum Schl based on the analysis of fatty acid biomakers Abstract: 【Objective】Characteristics of PLFA biomarkers in Fusarium oxysporum Schl. were study by means of microbe identification system (MIDI system from USA),【Method】 fungl came from different hosts（watermelon, banana and peanut）were detected by MIDI, homogeneity of fatty acid from fungl was analysed by clustering, the key fatty acids of Fusarium oxysporum Schl were acquired by PCA, The discriminant model of Fusarium oxysporum Schl was figured out by stepwise discriminant analysis.【Result】The results showed that 8 fat acids were identified in 26 strains,there were fat acids 16:0、18:0 and 18:2 CIS 9,12/18:0a in all strains. Using the cluster analysis method the 26 strains were classified into 4 groups based on the PLFAs biomarkers,and had no association with origin, biochemical variant and pathotype of Fusarium oxysporum Schl. The five key fatty acid obtained 14:0（X1），16:0(X2)，16:1 CIS 9 (W 7)(X3)，18:0(X4) and 18:2 CIS 9,12/18:0a(X5) were acquired by Principal Component Analysis (PCA). Bayes discrimination method was used to establish discriminant models. Four fatty acid of 14:0(X1), 16:0(X2), 18:0(X4), 18:2 CIS 9,12/18:0a (X5) were put into the model set as follow:Y1=-183.06+12.24X1+ 8.05X2+5.39X4+3.58X5，Y2=-174.09+10.40X1+7.96X2+5.40X4+3.42X5，Y3=-173.97+14.85X1+7.25X2+ 6.58X4+ 3.53X5. The accurate rate for discrimination was up to 90.00%.【Conclu