VEGF在人胚胎干细胞向神经元分化中作用的研究.pdfVIP

134 Stroke and Nervous Diseases, Jun 20 09 ,Vol. 16, No.3 VEGF 焦淑洁 许慧芳 许 杰 张苏明 湛彦强 ( vascular endothelial growth factor,VEGF) ,3 : A , B+ VEGF( 10 ng/ mL), C + VEGF( 10 ng/ mL) + VEGF 2/ Fc ( 10 ng/ mL) ; T-PC , T-PC OCT4 NestinM AP2 ; B AC , ( P 0 .0 1) , AC ( P 0. 05) ; 2 2 741 A 10 07-047 8( 20 09)03-0 134-04 Effect of VEGF on Neural Differentiation of Human Embryonic Stem Cells in vitro J iao S huj ie, X u H ui- f ang, X u J ie, et al . D ep artment of N eurol ogy , Tongj i H osp ital , H uaz hong University of S ien e and Te h- nology , Wuhan 43 0030 Abstract Objective To observe the effect of VEGF on neural differentiation of human embryonic stem cells(hESCs) in v itro. Methods After embryonic body formation, hESCs were differentiated into neural cells through selection and expansion procedures. hESCs were divided into three groups: group A, routine induc- tion; group B, routine induction+ 10 ng/ mL VEGF treated and group C, routine induction+ 10 ng/mL VEGF + 10 ng/ mL VEGF 2/ Fc treated. The cellular morphology was observed by contrast phase microscope. T he markers of every stage of induction were detected through T-PC . Nestin and M AP2 expressing cells were detected via immunofluorescence method. T he percentages of the imunostaining positive cells were determined by flow cytometer(FCM ) . esults OCT4 Nestin and MAP2 were detected by T-PC . The percentage of Nestin and MAP2 positive cells in group B were much higher than in group A and group C( P 0 .0 1) . There was no difference between group A and group C(P 0 .0 5) . The results of FCM were roughly similar. Conclu- sions VEGF, via VEGF 2 , stimulates the