原发性肝细胞癌中乙型肝炎病毒基因整合的突变分析_张婷.pdfVIP

2011 6 Jun. 2011 32 3 Journal of CapitalM ed icalU niversity Vol. 32 No. 3 [ do:i 10. 3969/j. issn. 2011. 03. 001] 原发性肝细胞癌中乙型肝炎病毒基因整合的突变分析 1 1 1 2 1 1* 1 张 婷 许 强 杨紫伟 蒋素贞 鲁凤民 陈香梅 庄 辉 ( 1. , 1001 1; 2. , 510515) ( hepatitis B virus, HBV) A luPCR LMPCR 40 H BV , PCR ABI 3700, PubM ed BLAST B ioEd it 65. 0% HBV ( 26/40), 37 H BV 67. 5% H BV ( 27/40), 68 HBV 1. 42, 2. 52, HBV A 1762T /G 1764A , HBV H BV , ; HBV A 1762T /G 1764A, ; ; ; R 512. 6 Identification of integrated hepatitisB vir sm tations in hepatocell lar carcinoma 1 1 1 2 1* ZHANG T ing , XU Q iang, YANG Ziw ei, JIANG Suzhen , LU Fengm in , 1* 1 CHEN X iangm ei , ZHUANG Hui ( 1. D ep artm ent of M icrobiology, Peking Univers ity H ealth Science Cen ter, B eij ing 1001 1, Ch ina; 2. D ep artm ent of Inf ectiousD iseases, N anf ang H osp ital, SouthernM ed ical Un iversity; Guangzhou 510515, China) Abstract Objective To investigate the relevance of the basic core prom oter( BCP) mu tants of integrated hepatitis B virus( HBV) in liver tissues from patientsw ith hepatocellular carcinom a( HCC). M ethods A lu repeat sequence polym erase chain reaction (A luPCR ) and ligationm ed iated( LM ) PCR w ere used to identify theH BV integration in 40 pairs of HBVrelated H CC tissues and adjacent non tumorous liver tissues. The PCR productsw ere purified and subjected to d irect sequencing by AB I 3700 Auto sequencer. NCB I BLAST and B io Ed it software w ere used for analysis ofHBV sequence. R es lts In 40 H BsAg positiveHCC samp les, 26( 65. 0% ) showed HBV integration in host genom e, w i