Solexa测序原理及流程.pptVIP

Solexa测序原理及流程 深圳华大基因研究院 Agenda DNA样品制备 Cluster Formation Binding to flowcell Bridge Amplification SBS (Sequencing By Synthesize) One Cycle One Base Pair End DNA样品制备 Cluster formation Cluster FormationBridge Amplification Cycle DNA synthesization Sequencing By Synthesization 三种特殊序列 P7序列：flowcell上结合的序列，边合成边边测序（SBS）过程模板序列的5’端。 P5序列：flowcell上结合的序列。 SBS引物序列(oligo) Bridge Amplification Pair End Different Cluster template Specification comparison: THE END! THANKS! * * P7 P5 SBS oligo Fragment DNA 5’ 3’ 5’ + 3’ 5’ 5’ + 5’ 3’ 3’ 5’ 1.End repair T4 polymerase , DNA Pol 1 (Klenow fragment) 3’ 5’po4 po