筛分树脂对三七皂甙中人参皂甙Rb1分离研究.pdfVIP

筛分树脂对三七皂甙中人参皂甙Rb1的分离研究 武春密，陈艳立，王春红，施荣富∗ (功能高分子材料教育部重点实验室, 南开大学高分子化学研究所, 天津, 300071) 摘要：三七总皂甙中的主要单体皂甙Rb1具有特殊的药理药效，也是制备高活性次生甙的原料，所以从三七 皂甙中分离单体人参皂甙Rb1引起了人们越来越多的关注。三七皂甙中几种主要单体皂甙的结构相似，极性 差别不大，用一般大孔树脂难以分离。但是由于Rb1在四种单体皂甙中分子尺寸最大，受到筛分分离机制的 启发，我们期望合成孔径较小且均匀、在保持传统吸附树脂高吸附容量的基础上具有分子尺寸精确筛分能力 的新型孔结构树脂。本文研究了树脂孔径调控方法，考察了不同孔径的树脂对单体皂甙的分离效果，结果表 明，在筛分和吸附的协同作用下，树脂对Rb1 有很好的分离能力，只经过一步吸附，即可将Rb1 与其他单体 皂甙完全分离，Rb1 在三七总单体皂甙中的比例从31.2 ％提高到95.6 ％。 关键词：三七皂甙；人参皂甙 Rb1 ；吸附树脂；筛分 Isolation for Rb1 from Panax Notoginosides with Polymeric Adsorbent Based on the Sieving Effect WU Chun-mi, CHEN Yan-li, WANG Chun-hong, SHI Rong-fu* (The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China) Abstract: San qi has been used for thousands of years as a traditional medicine processing central nervous system-stimulating, cardiotonic and hypotensive effects. The active components are panax notoginsenosides (PNS), and the most abundant components present in Panax Notogenseng are Rb1, Rg1, Re and notoginsenoside R1. The individual compounds nearly have the same biological activity as PNS ，but some of the individual compounds have special biological effect. Along with the gradual in-depth study, It has been found that some monomers have great differences in pharmacology effect, and evenly opposite. And according to the recent reports, several 收稿日期： ∗联系人：施荣富（1945-），男，教授，主要从事吸附分离功能高分子材料合成及天然产物提取应用研究工作 E-mail：srf2004@nankai.edu.cn 第一作者：武春密（1982- ），女，硕士研究生，E-mail：wuchunmi333@nankai.edu.cn rare ginsenosides commonly possess obvious pharmacology effect, Such as Rh2 can reduce the proliferation of a variety of cultured cancer cells. Because these rare ginsenosides are minor ginsenosides so that the isolation of them in a pure state fro