因芯片技术研究进展.pdfVIP

安徽农业科学袁允燥怎则灶葬造 燥枣 粤灶澡怎蚤 粤早则蚤援 杂糟蚤援 园园7袁35渊8冤院2241-2243 袁2245 责任编辑 姜丽 责任校对 左佳 因芯片技术研究进展 王洪水，侯相山 （东营职业学院，山东东营 257091 ） 摘要 主要就基因芯片的概念、技术原理、分类、主要技术流程、应用及其存在的问题和应用前景作了综述。 关键词 基因芯片；基因组研究；基因诊断；技术流程 中图分类号 文献标识码 文章编号 （ ） Q78 A 0517-6611 2007 08-02241-03 Research Progress of Gene Chip Technology WANG Hong shui et al (Dongying Vocational College, Dongying, Shandong 257091) Abstract Gene chip was a newly developed technology in recent years. Light 蛳directed in situ oligonucleotide synthesis or microarray printing technique was adopted in its basic process in order to generate arrays of large amounts of probes on the surface of small substrates such as silicon ， chips, which was hybridized to the amplified and labeled samples. After scanning and analyzing the signals provided hereditary information on the samples. This technology could not only be used to qualitative analysis or quantitative analysis of hereditary information, but also could be used to genome study and gene diagnosis. Concept, technique mechanism, category, main technique process, applications, existent problems and its application foreground of gene chip were summarized in this review. Key words Gene chip; Genome research; Gene diagnose; Technique process 世纪 年代初，人们根据计算机半导体芯片制作 有序地、高密度地排列在一块 2 大小的玻片或胶片 20 80 1耀2 cm 技术将晶体管集成在芯片上，并提出将寡核苷酸分子也集 上，形成可与目的分子相互作用的固相表面，然后与标记的 [1] 成在芯片上的设想 。 等将短的 片段固 在支持 样品进行杂交，通过检测杂交信号的强度及分布进行分析。 Bains DNA [2] 物上，通过杂交进行序列分析，做了有益的尝试 。从Schena 它与其他分析基因