基于纳米金的限制性内切酶比色探针.pdfVIP

基于纳米金的限制性内切酶比色探针 金叶倩 付静 屠一锋 严吉林* 苏州大学化学系 苏州工业园区仁爱路199 号，苏州 215123 *Email：yanjl@suda.edu.cn ， Tel 在本工作中，我们构建了用于限制性内切酶传感的比色探针。探针由两种 DNA 修饰的金纳米组成，限制性内切酶识别并水解位于其中一个纳米粒子上的 特定双链DNA ，使残留单链DNA 片段，该DNA 片段可与位于另一纳米金表面 的单链DNA 杂交，从而导致纳米金的聚集。由于纳米粒子尺寸的变化导致吸收 光谱的红移从而产生明显的颜色变化，可通过肉眼观察即可对限制性内切酶进行 半定量测定。通过对紫外-可见吸收光谱的考察可获得定量的信息。 参考文献 1. Nidhi Nath and Ashutosh Chilkoti Anal. Chem., 2002, 74 (3), pp 504–509 2. Weian Zhao, M. Monsur Ali, Sergio D. Aguirre, Michael A. Brook and Yingfu Li Anal. Chem., 2008, 80 (22), pp 8431–843 A two-nanoparticle colorimetric probe for restriction endonuclease recognition Yeqian Jin, Jing Fu, Yifeng Tu, Jilin Yan Department of chemistry, Suzhou University 199 Ren’ai Road, Suzhou 215123 Email: yanjl@suda.edu.cn Tel: Abstract In this work we reported a two-nanoparticle probe for recognition and colorimetric detection of a restriction endonuclease. Recognition and hydrolysis of double stranded DNAs (dsDNA) on one gold nanoparticle by the enodonuclease leaves single strand DNAs only on the surface, which further hybridize with another single strand DNAs from the other gold nanoparticle. This hybridization holds the two nanoparticles together, a color change shows up due to the changes in particle size and related localized surface plasma resonance. The probe is simple in both design and application, no extra instrumentation is required for qualitative and semi-quantitative results. Detailed quantification is also obtained with a UV-vis spectrometer. References 1. Nidhi Nath and Ashutosh Chilkoti Anal. Chem., 2002, 74 (3), pp 504–509 2. Weian Zhao, M. Monsur Ali, Sergio D. Aguirre, Michael A. Brook and Yingfu Li Anal. Chem., 2008, 80 (22), pp 8431–843