N乙酰半胱氨酸对砷致小鼠脂质过氧化损伤的保护作用研究.pdfVIP

N-乙酰半胱氨酸对砷致小鼠脂质过氧化损伤的保护作用研究 向金梅1,2 郭定宗1 杨世锦1 （1 华中农业大学动物医学院 湖北 武汉 430070 ） （2 湖北生物科技职业学院 湖北 武汉 430070 ） 摘要：目的 探讨 N-乙酰半胱氨酸(NAC) 对砷致小鼠脂质过氧化损伤的拮抗作用。方法 40 只昆明小鼠随机分为 5 组：对照组，单纯染砷组，低、中、高剂量NAC处理组。染毒8周后处死小鼠，取其血液和肝、肾，并检测肝、肾抗氧 化酶SOD、GSH-Px、CAT活性和脂质过氧化产物MDA含量及血SOD、δ-氨基酮戊酸脱水酶(δ-ALAD)活性和MDA、Hb含量。 结果 与对照组相比，单纯染砷组小鼠抗氧化酶活性显著降低(P0.01)，MDA含量显著增加(P0.01) )；低、中剂量NAC 处理后可有效提高机体抗氧化酶活性(P0.01或P0.05)，减少脂质过氧化产物MDA 含量(P0.01或P0.05)。 结论 适 量NAC能有效抑制砷所致自由基的产生和脂质过氧化反应，提高机体抗氧化酶活性，从而发挥其对机体的保护作用。 关键词：砷；N-乙酰半胱氨酸；抗氧化酶；脂质过氧化 Study on the protective effect of N-acetyl-cysteine and VitE on lipid peroxidation damage induced by arsenic in rat 1 Xiang Jinmei Guo Dingzong Yang Shijin (1 College of animal veterinary, Huazhong agricultural university, Wuhan, Hubei, China) （2 College of Hubei biotechnology, Wuhan, Hubei, China ） Abstract ：The aim of the experiment was to study the antagonistic action of N-acetyl-cysteine on the lipid peroxidation damage induced by arsenic in blood, liver and kidney of rats. 40 Kunming rats were divided into five groups: control, arsenic exposed group, NAC groups of low dose, medial dose and high dose. Eight weeks later, all rats were killed to examine the changes of some biochemical indices (the antioxidase SOD, GSH-Px, CAT and the production of lipid peroxidation MDA in liver and kidney and SOD, δ-ALAD, Hb, MDA in blood).The results showed as fellows: (1) Compared with those of control, the antioxidases activity of arsenic exposed group was significantly lower (P0.01); and the MDA content was significantly higher (P0.01). (2) Compared with those of arsenic exposed group, the antioxidases activity increased significantly (P0.05 or P0.01), and the