Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method.pdfVIP
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Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method.pdf
CHIN.PHYS.LETT.Vo1.27,No.10(2010)107801
Label-Freeand H igh—ThroughputDetection ofProtein M icroarraysby
Oblique—IncidenceReflectivity D ifferenceM ethod
WANGxu(王旭) ,LuHeng(~ ),WENJuan(~ ),YUANKun(原昆),L0Hui—Bin(吕惠宾)
JINKui—Juan(~ 娟),ZHOUYue—Liang(N岳亮),YANGGuo—Zhen(杨国桢),
BeijingNationalLaboratoryforCondensedMatterPhysics,InstituteofPhysics,ChineseAcademyofSciences
Beijing100190
。SchoolofScience Jiangnan University,Wuxi214122
,
(Received25January2010)
Ⅵ,elabel—freedetected the biologicalprocessofpreparing amicroarray thatincludes400 spotsofmouseim—
munoglobulinG (IgG)aswellasthespecifichybridizationbetweenmouseIgGandgoatanti—mouseIgGbyan
oblique—incidencereflectiyit ) . 一
. ydifference(OI-RD method Thedetectionresultsaftereachprocessincludingprint
ing,washing,blocking.andhybridization,demonstratethatthe0 RD methodcantracethepreparationprocess
ofamicroarrayand detectthespecifichybridization between antigensandantibodies.0LRD isapromising
.
methodofrlabel-freeandhigh—throughputdetection ofbiologicalmicrorarays.
PACS:78.20.Ci.42.79.Ls DOI:10.1O88/o256—3o7x/27/10/107801
InthepasttwentYyears,microarrays[1Jhavebeen immunoglobulinG (IgG)microarray,includingfour
wildly used in biologicalexperim entssuch asgene steps:printing,washing,blockingand hybridization.
expression,DNA replication,proteintrafficking,and TheexperimentalresultsdemonstratethatOI—RD is
proteomicsetc.[2,31Becauseoftheirhigh spotinten— afeasiblem ethodtola
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