茶红素清除DPPH自由基能力地研究.pdfVIP

全国茶业科技学术研讨会论文集 茶红素清除 DPPH 自由基能力的研究 * 王 华，李大祥 ，宛晓春，魏志文 （安徽农业大学教育部/农业部茶叶生物化学与生物技术重点实验室 合肥 230036 ） 摘要：茶红素是一类异质的酸性酚性色素的总称，在红茶中含量极为丰富,有关其结构、 组成及生物学活性等理化性质也都不甚清晰。本文以 DPPH 自由基为对象，探讨了祁 门红茶中不同极性的茶红素部分对其清除能力。结果表明，五种不同极性的茶红素部 分对 DPPH 自由基均有一定的清除能力，并呈现一定的量效关系。在浓度为 66.7 µg/ml ， 五种不同极性的茶红素部分清除率均达 85% 以上。在 13.2∼44.4µg/ml 范围内，随着茶 红素部分极性的增加，其对 DPPH 自由基的清除能力总体上呈逐渐下降的趋势。 关键词：茶红素；分级分离；DPPH 自由基 Studies on the DPPH radical scavenging activity of thearubigins WANG Hua, LI Daxiang, WAN Xiaochun, Wei Zhiwen (Key Laboratory of Tea Biochemistry Biotechnology of Ministry of Agriculture and Education, Anhui Agricultural University, Hefei230036) Abstract: Thearubigins are heterogeneous acidic phenolic pigments, which are the most abundant polyphenols in black tea. Unlike well-characteristic catechins and theaflavins in tea, thearubigins are poorly characterized. In order to explore their biological effects, five different polar thearubigins (TRs-1～TRs-5) were prepared with fractionated percipitaion from Keemun black tea and their DPPH radical scavenging activity were assayed in vitro. The results showed that all of five parts could scavenge the free radical in a dose-response manner. At 66.7 µg/ml, each of them scavenged more than 85% of DPPH radicals. Moreover, the scavenging ratio decreased with the increase of the polarity of thearubigin fractions at the range of 13.2∼44.4µg/ml. Those results indicated that thearubigins contributed to the total anti-oxidative effects of black tea. Keywords: Thearubigins; fractionated precipitation; DPPH radical 茶鲜叶中含有大量的多酚类化合物，占其干重的 18%～36% 。鲜叶中多酚主要 为儿茶素（黄烷-3-醇），其占多酚总量的 80%左右。在红茶加工过程中，茶鲜叶 的儿茶素在内源多酚氧化酶和过氧化物酶的作用下，氧