胞二磷胆碱预处理对大鼠脊髓缺血再灌注损伤的影响.docVIP

胞二磷胆碱预处理对大鼠脊髓缺血再灌注损伤的影响 曾小华，刘丹彦，安 民 （400016 重庆，重庆医科大学附属第一医院麻醉科） [通信作者] 刘丹彦，E-mail：liudanyan418@ [摘要] 目的 探讨胞二磷胆碱对脊髓缺血再灌注损伤的影响及其作用机制。方法 雄性SD大鼠72只，体质量（230±20）g，按随机数字表法分为3组（n=24）：假手术组，缺血再灌注损伤组（IR组）和胞二磷胆碱预先给药组（citi组），各组按灌注后12、24、48、72 h分成4个亚组（n=6）。各组均采用改良Zivin法建立脊髓缺血再灌注损伤模型，并于灌注后12、24、48、72 h 4个时点采用改良Tarlov法对大鼠脊髓运动和感觉功能进行评分，HE染色观察脊髓L2-L5节段的病理学改变，TUNEL法检测脊髓凋亡细胞数，于再灌注后72 h处采用免疫组化方法检测脊髓神经元Bcl-2、Bax、caspase-9表达及Bcl-2与Bax的比值（Bcl-2/Bax）。结果 与假手术组相比，IR组和citi组运动和感觉评分均降低，HE染色有明显的病理损伤，TUNEL染色神经细胞凋亡数量增加，免疫组化显示caspase-9 、Bcl-2、Bax蛋白表达增加，Bcl-2/Bax降低（P＜0.01）；与IR组相比，citi组运动和感觉评分高，HE染色形态学损伤较轻，TUNEL染色神经细胞凋亡数量少，免疫组化Bcl-2表达明显增加，Bax、caspase-9表达降低，Bcl-2/Bax升高（P＜0.01）。结论 胞二磷胆碱可减轻大鼠脊髓缺血再灌注损伤，改善神经功能，其机制可能与其上调Bcl-2蛋白表达，下调Bax、caspase-9蛋白表达，从而减轻神经元凋亡有关。 [关键词] 脊髓；胞二磷胆碱；预处理；缺血再灌注损伤；凋亡 [中图法分类号] [文献标志码] A Effects of citicoline pretreatment on spinal cord ischemia-reperfusion injury in rats Zeng Xiaohua , Liu Danyan , An Min (Department of Anesthesiology, First Affiliated Hospital , Chongqing Medical University , Chongqing , 40016,China ) [ABSTRACT] Objective To investigate the effects of citicoline pretreatment on spinal cord ischemia-reperfusion (IR) injury in rats and the mechanism involved. Methods seventy-two male SD rats weighing 210~250g were randomly divided into 3 groups (n=24): sham operation group, ischemia-reperfusion injury (group IR) and citicoline pretreatment (group citi). Every group was divided into four subgroups at reperfusion 12、24、48、72 h(n=6). Using Zivin improvements methods to establish model, then all treated rats were randomly divided into four subgroups at reperfused 12h、24h、48h、72h for neurological function scores with Tarlov improvements methods, HE staining for L2-5 spinal cord pathological changes, calculation apoptosis cells with TUNEL method，and using immunohistochemical assay for Bcl-2、Bax、caspase-9 expression levels ，the ratio of Bcl-2 and Bax (Bcl-2/ Bax) at reperfused 72h. Results Compared to group Sham, the neurological function scores of group IR