芪丹通脉片对大鼠心肌线粒体损伤的保护作用_医学论文.docVIP

芪丹通脉片对大鼠心肌线粒体损伤的保护作用_医学论文 芪丹通脉片对大鼠心肌线粒体损伤的保护作用_医学论文 作者：王宗仁，马静，行利，马世平，陈晓莉，樊爱琳，李晶华，肖铁卉，郑谨 【关键词】 芪丹通脉片 　　【Abstract】 AIM: To study the protective effects of Qidantongmai tablet (QDTMT) on the injury of myocardial mitochondrion induced by isoproterenol. METHODS: Rats were fed with QDTMT（2 g#12539kg-1）for two weeks and acute myocardial ischemia was induced by subcutaneous injection of isoproterenol (ISP. 5 mg#12539kg-1) into rats. The content of MDA and the activity of SOD, GSHPX and SDH in myocardial mitochondrion were measured. RESULTS: In the model group, the content of MDA increased and the activity of SOD decreased significantly. Compared with those in the model group, the MDA content of mitochondrion reduced in the QDTMT group and the activity of SOD, GSHPX and SDH increased significantly ［( 8.9±0.6 0) vs (5.5±0.6) μmol#12539 g-1, (673.2±25.2 ) vs (536.2±20.3) kNU#12539 g-1, (55.3±6.0) vs (40.7±7.1) kU#12539g-1, (0.07±0.01) vs (0.05±0.01) kat#12539g-1 , 0.01). CONCLUSION: QDTMT can alleviate the injury caused by oxygen free radicals to myocardial mitochondrion by decreasing the content of MDA and increasing the activity of SOD, GSHPX and SDH in myocardial mitochondrion. 　　【Keywords】 Qidantongmai tablets acute myocardial ischemia mitochondrion malondialdehyde superoxide dismutase glutathion peroxidase succinate dehydrogenase 　　【摘要】 目的： 探讨芪丹通脉片（QDTMT）对异丙肾上腺素（Iso）所致急性心肌缺血的防治作用. 方法： 给大鼠灌服QDTMT（2 g#12539kg-1） 2 wk，皮下注射异丙肾上腺素（Iso， 5 mg#12539kg-1），诱导急性心肌缺血模型，测定心肌线粒体（Mit）中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GXHPX）及琥珀酸脱氢酶（SDH）. 结果: 模型组线粒体中MDA含量增加，QDTMT组线粒体MDA含量显著低于模型组 ［ (8.9±0.6) vs (5.5±0.6) μmol#12539 g-1 , 0.01］；线粒体中模型组SOD活性降低，QDTMT组SOD活性升高，两者相比较有显著性差异 ［(673.2±25.2) vs (536.2±20.3) kNU#12539g-1 , 0.01］. QDTMT组GSHPX活力显著高于模型组 ［(55.3±6.0 ) vs (40.7±7.1 ) kNU#12539g-1 , 0.01 ］. QDTMT组线粒体中SDH活性明显升高 ［(0.07±0.01) vs (0.05±0.01) kat#12539g-1, 0.01］. 结论： QDTMT可通过降低心肌线粒体中MDA含量，增加SOD, GSHPX及SDH活力，减轻氧自由基对心肌线粒体的损伤. 　　【关键词】 芪丹通脉片；急性心肌缺血；线粒体；丙二醛；超氧化物歧化酶；谷胱甘肽过氧化物酶；琥珀酸脱氢酶 　　0引言 　　氧自由