荭草花水溶性化学成分的研究_药学论文.docVIP

荭草花水溶性化学成分的研究_药学论文 荭草花水溶性化学成分的研究_药学论文 作者：李勇军，何迅，刘志宝，周雯，兰燕宇，王爱民，王永林 【摘要】 目的研究荭草Polygonum orientale花活性部位的化学成分。方法采用溶剂提取，应用色谱技术分离纯化，根据化合物理化性质和光谱数据鉴定其结构。结果分离并鉴定了6个化合物，分别为N-顺式-对羟基苯乙基阿魏酰胺(N-cis-feruloyltyramine, 1)；N-反式-对羟基苯乙基阿魏酰胺(N-trans-feruloyltyramine, 2)；N-p-香豆酰酪胺(paprazine, 3)；5,7-二羟基色原酮(5,7-dihydroxychromone, 4)；3,5,7-三羟基色原酮(3,5,7-trihydroxychromone, 5)；5,7,4′-三羟基二氢黄酮醇(5,7,4′-trihydroxy-dihydroflavonol, 6)。结论化合物1～3为首次从该种植物中分离得到。 【关键词】 荭草； 化学成分； 结构鉴定 Chemical constituents of Flowers of Polygonum orientaleLI Yongjun, HE Xun, LIU Zhibao, ZHOU Wen, LAN Yanyu, WANG Aimin, WANG YonglinSchool of Pharmacology, Guiyang Medical College,Guiyang 550004, ChinaAbstract：ObjectiveTo study the chemical constituents of the essential substance from the flowers of Polygonum oriental.MethodsChromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence. ResultsSix compounds were obtained and identified as N-cis-feruloyltyramine (1), N-trans-feruloyltyramin (2), paprazine (3), 5,7-dihydroxychromone (4), 3,5,7-trihydroxychromone (5), 5,7,4′-trihydroxy-dihydroflavonol (6).ConclusionCompounds 1-3 were isolated from Polygonum oriental for the first time. 　　Key words：Polygonum oriental; Chemical constituents; Structure identification 荭草花为蓼科植物荭蓼Polygonum orientale L.的花序，又名水荭花，何草花，狗尾巴花；荭草花具有行气活血、消积、止痛的功效，用于治疗头痛、心胃气痛、腹中痞积等［1］。课题组在对荭草各部位药理筛选实验中发现荭草花水煮醇沉后的正丁醇萃取部位有较强的抗心肌缺血，减少心肌梗塞面积作用。为阐明其活性成分，作者对荭草花水煮醇沉后的正丁醇萃取部位进行了化学成分研究，在前期研究中已分离出9个化合物，在对其进一步的研究中作者又分离得到了6个化合物：N-顺式-对羟基苯乙基阿魏酰胺 (N-cis-feruloyltyramine, 1)；N-反式-对羟基苯乙基阿魏酰胺 (N-trans-feruloyltyramine, 2)；N-p-香豆酰酪胺 (paprazine, 3)；5,7-二羟基色原酮 (5,7-dihydroxychromone, 4)；3,5,7-三羟基色原酮(3,5,7-trihydroxychromone, 5)；5,7,4′-三羟基二氢黄酮醇(5,7,4′-trihydroxy-dihydroflavonol, 6)。其中化合物1～3为首次从该种植物中分离得到，其结构式见图1。 　　1 仪器与材料 JEOL ECX 500MHz核磁共振波谱仪，HP-LC-MSD电喷雾质谱仪，VECTOR-22红外分光光度计，Agilent1100半制备高效液相色谱仪。色谱用硅胶（200～300目）及硅胶G预制板为青岛海洋化工厂生产，聚酰胺（30～60目）为浙江黄岩化工厂生产，C18反相硅胶