蛋白质芯片与ELISA法对肿瘤标志物检测结果的对照研究_药学论文.docVIP

蛋白质芯片与ELISA法对肿瘤标志物检测结果的对照研究_药学论文 蛋白质芯片与ELISA法对肿瘤标志物检测结果的对照研究_药学论文 【摘要】 目的 研究蛋白质芯片与ELISA法对肿瘤标志物检测结果的差异。方法 分别应用蛋白质芯片和ELISA法测定分析50例PHC患者、17例肝硬化患者、16例肝炎患者和40例健康查体者血清中CA199、AFP和CEA的水平。结果 采用蛋白芯片联合检测CA199、AFP及CEA等3项指标对PHC的诊断阳性率为78.00%，特异性为82.19%；而采用ELISA法结果为78.00%和75.34%，两者之间差异无统计学意义（P＞0.05）。两种方法检测结果符合率为92.95%（相关系数r=0.842, P＜0.001）。结论 蛋白质芯片能够较准确地反映肿瘤标志物的水平，并且较传统方法快速方便，可以作为检测标志物的常规手段之一。 【关键词】 蛋白质芯片; ELISA; 原发性肝癌; 肿瘤标志物 　　Abstract：Objective To evaluate the detected difference of tumor markers between protein biochip and ELISA method.Methods The serum levels of 3 common used tumor markers, including AFP, CA199, and CEA, were measured with the C12 protein biochip detective system in 50 primary hepatic cancer patients, 17 patients with liver cirrhosis, 16 patients with chronic hepatitis and 40 healthy persons. Meanwhile, the 3 tumor markers serum levels were also detected by ELISA.Results Combined measured positive rate and specificity for PHC were 78.00% and 82.19% when the 3 tumor markers were measured with protein biochip, but the positive rate and specificity were 78.00% and 75.34% by ELISA. There wasnt significant difference between these two methods（P＞0.05）.The coincident rate was 92.95%, and spearman rank correlation was 0.842(0.001).Conclusion Protein biochip could be measured the serum tumor markers levels accurately, and it was more quickly and conveniently than traditional methods. It could be used as a common means for the measurement of tumor markers. 　　Key words：Protein biochip; ELISA; Primary hepatic cancer; Tumor markers 　　0 引言 　　蛋白质芯片是一种高通量、高灵敏度、高特异性且微型化的蛋白质分析技术[1]。实验表明，该项技术对多种疾病的早期诊断均有一定的作用[2,3]。酶联免疫吸附试验（Enzymelinked immunosorbent assay, ELISA）是一种常用于检测蛋白质的传统方法。本实验以原发性肝癌（primary hepatic cancer，PHC）为例，分别应用蛋白质芯片与ELISA法检测PHC患者血清中甲胎蛋白（alpha， AFP）、糖类抗原19（Carbohydrate antigen 19，CA199）和癌胚抗原（Carcinoembryonic antigen，CEA）的水平，分析不同方法下检测结果的差异，并对两种方法的相关性进行研究。 　　1 材料与方法 　　1.1 检测对象 　　PHC组为本院2003年1月～7月住院患者，均经病理检查确诊为肝细胞癌，包括Ⅰ期4例，Ⅱ期5例，Ⅲ期10例，Ⅳa期22例，Ⅳb期9例；其中男36例，女14例，平均年龄53