血清蛋白质谱模型对胃腺癌诊断的应用性研究_医学论文.docVIP

血清蛋白质谱模型对胃腺癌诊断的应用性研究_医学论文 血清蛋白质谱模型对胃腺癌诊断的应用性研究_医学论文 【摘要】 　　［目的］探讨用蛋白质芯片技术筛选胃腺癌患者血清蛋白质表达谱,寻找血清中的标志性蛋白。［方法］采用蛋白质生物芯片表面增强激光解析电离飞行时间质谱（SELDI）技术,运用SAX2 (Strong Anionic Exchanger)蛋白质芯片检测胃腺癌患者、胃炎患者和健康者血清,建立诊断模型,然后进行单盲模型验证。［结果］发现5910Da、5084Da和8691Da的三个蛋白质荷比峰(M/Z)在胃腺癌和健康组比较中具有显著性差异。5910Da、6440Da的两个蛋白质荷比峰(M/Z)在胃腺癌和胃炎组中比较具有显著性差异。［结论］建立了胃腺癌的血清蛋白指纹质谱，为以后的胃癌蛋白质组学研究奠定了一定的基础，建立了以5910Da、5084Da、8691Da和6440Da四个蛋白质峰为模型区分胃腺癌与非胃腺癌的血清蛋白表达质谱诊断模型，为胃腺癌的临床诊断提供了一条崭新的途径和方法。 【关键词】 表面增强激光解析电离-飞行时间质谱　蛋白质芯片　生物标记　胃腺癌 　　Application of Serum Proteomic Patterns for Detection of Gastric Adenocarcinoma serum protein profiling ofastricadenocarcinoma by surface-enhancedlaser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS) to discovering the discriminalory proteins.［Methods］The diagnosis model was established by SELDI technique with SAX2 chip to detect the serum of patients with gastric adenocarcinoma, gastricism and healthy persons, and tested by single-blind model.［Results］Three specific protein fingerprint biomarkers massed at 5910, 5084, 8691 between gastric adenocarcinoma group and control group with statistical significance. Two specific protein fingerprint biomarkers massed at 5910, 6440 between gastric adenocarcinoma group and gastricism group with statistical significance.［Conclusions］ A gastric adenocarcinoma protein fingerprint and a foundation for later study of gastric cancer proteomics is developed. The diagnostic model of the protein profiling(5910Da, 5084Da, 8691Da, 6440Da) for distinguishing gastric adenocarcinoma from non-gastric adenocarcinoma is established to aid a new approach in clinical diagnosis for gastric adenocarcinoma. 2.Institute of Cell Biology, Zhejiang University, Hangzhou 310031,China)Abstract: ［Purpose］ Toscreen out 胃腺癌是常见的癌肿之一，占全部恶性肿瘤的第三位，占消化道恶性肿瘤之首,而且近年来发病有明显年轻化趋势。相当多的胃腺癌患者发现时已经是晚期，我国每年死于胃腺癌约16万人。目前已有的肿瘤标志物单项指标检测胃腺癌的灵敏度仅为18%~40%，联合应用的结果虽然可达60%~80%，但假阳性率较高，难以用于早期诊断。因此，寻求早期诊断胃腺癌的特异方法是目前临床亟待解决的难题。我们利用表面加强激光解吸离子化飞行时间质谱（surface?鄄enhanced laser desorpt