血清HBV-DNA与HBV免疫学标志物之间的相关性研究_医学论文.docVIP

血清HBV-DNA与HBV免疫学标志物之间的相关性研究_医学论文 血清HBV-DNA与HBV免疫学标志物之间的相关性研究_医学论文 蔡 晋，李爱军，向梦生，吴丽娟，夏 季　　　　　　　　　　　　　　　　　【摘要】 目的 研究乙肝血清HBV-DNA与HBV免疫学标志物之间的相关性。方法 采用双盲实验，血清HBV-DNA采用Real-time PCR进行定量检测，血清免疫标志物采用ELISA法。结果 在344份血清标本中，HBV五项免疫学标志物出现11种组合，主要有以下5种，组合Ⅰ为HBsAg（+）、HBeAg（+）、HBcAb（+）88份，HBV-PreS1阳性率为68.18%，血清HBV-DNA阳性率为96.59%；组合Ⅱ为HBsAg（+）、HBeAb（+）、HBcAb（+）144份，HBV-PreS1阳性率为56.94%，血清HBV-DNA阳性率为50.69%；组合Ⅲ为HBsAg（+）、HBcAb（+）36份，HBV-PreS1阳性率为55.56%，血清HBV-DNA阳性率为63.89%；组合Ⅷ为HBsAb（+）41例，HBV-PreS1全部阴性，血清HBV-DNA阳性率为26.83%；组合Ⅺ为HBV-M（-）17例，HBV-PreS1全部阴性，血清HBV-DNA阳性率为11.76%。HBV的ELISA阳性模式Ⅰ、Ⅱ、Ⅲ与荧光定量PCR结果进行独立性检验、差异性检验P值均＜0.05，即HBV的ELISA阳性模式Ⅰ、Ⅱ、Ⅲ与荧光定量PCR结果之间存在关联性，且荧光定量PCR结果阳性率高于HBV的ELISA阳性模式。结论 血清HBV-DNA更能反映HBV复制的程度，其载量与抗原存在呈正相关性；HBV-DNA检测对乙肝早期诊断、病毒复制水平判断和疗效监测等均具有重要的临床意义。 【关键词】 HBV-DNA；Real-time PCR；ELISA；HBV-M；HBV-PreS1 【Abstract】 Objective To study the relationship between serum HBV-DNA and six types immune markers for hepatitis B virus (HBV-M).Methods Serum HBV-DNA was determined by real-time PCR assay while the six types HBV-M by ELISA method.Results Of 88 patients positive for HBsAg，HBeAg and HBcAb，the HBV-PreS1 positive rate was 68.18%，and the HBV-DNA positive rate was 96.59%.Of 144 patients positive for HBsAg，HBeAb and HBcAb，the HBV-PreS1 positive rate was 56.94%，and the HBV-DNA positive rate was 50.69%.Of 36 patients positive for HBsAg and HBcAb，the HBV-PreS1 positive rate was 55.56%，and the HBV-DNA positive rate was 63.89%.Of 41 patients positive for HBsAb，the HBV-PreS1 positive rate was 0，and the HBV-DNA positive rate was 26.83%.Of 17 patients negative for the HBV-M，the HBV-PreS1 positive rate was 0，and the HBV-DNA positive rate was 11.76%.The results of HBV-DNA fluorogenic quantitative PCR were significantly correlated with the positive models Ⅰ，ⅡandⅢ of HBV(0.05)，and the positive rates of HBV-DNA fluorogenic quantitative PCR were significantly higher than the rates of positive models Ⅰ，Ⅱand Ⅲ of HBV(0.05).Conclusion There was a positive good correlation between carrying capacity of