大黄蒽醌类成分的提取方法筛选及均匀设计优化工艺研究.pdfVIP

大黄蒽醌类成分的提取方法筛选及均匀设计优化工艺研究 1＊ 2 1# 崔红花 ，赵英日，王淑美 （1.广东药学院中药学院，广州市 510006；2.广州中医药大学国际学院，广州市 510405） 中图分类号 R283；R284 文献标识码 A 文章编号 1001- 0408（2010）07- 0612- 03 摘 要 目的：研究大黄中蒽醌类成分的最佳提取方法并优化其提取工艺。方法：分别用超声法、加热回流法和索氏提取法对大黄 进行提取，以总蒽醌、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量为指标比较3种提取方法。在此基础上通过均匀设 计法研究总蒽醌（UV ）、5种蒽醌类化合物的提取工艺。结果：加热回流法为最佳提取方法。最佳提取工艺为提取时间90min 、提取 次数1次、甲醇浓度95％、药材目数2.0007目，蒽醌类成分综合评分可达6.556分。结论：该提取工艺设计合理，质量易于控制。 关键词 大黄；蒽醌类成分；加热回流法；均匀设计法 Technology for Optimizing Extraction Method and Uniform Design of Anthraquinones Components of Radix et Rhizoma Rhei CUI Hong-hua ，WANG Shu-mei （College of TCM ，Guangdong Pharmaceutical University ，Guangzhou 510006， China ） ZHAO Ying-ri （College of International Education ，Guangzhou University of TCM ，Guangzhou 510405，China ） ABSTRACT OBJECTIVE ：To study extraction method of anthraquinones components of Radix et Rhizoma Rhei and optimize extraction technology. METHODS ：Ultrasonic extraction ，heating reflux extraction and soxhlet extraction were adopted to extract Radix et Rhizoma Rhei respectively. 3 kinds of extraction technology were compared with the content of total anthraquinone ， aloe- emodin ，rhein ，emodin ，chrysophanol ，physcione as index. The extraction technologies of UV and 5 kinds of anthraquinone compounds were optimized by uniform design method on the basis of previous study. RESULTS ：Heating reflux extraction which was optimal extraction technology was as follows ：extracting time of 90 min ，extracting for 1 time ，methanol concentrations of 95％，medicinal mesh of 2.000 7. Comprehensive score of anthraquinones could reach 6.556.CONCLUSION ：The extraction technology is reasonable ，available in quality control. KEY WORDS Radix et Rhizoma Rhei ；Anthraquinones components ；Heating reflux extraction ；Uniform design 大黄是萝科植物掌叶大黄Rheum palmatum L. 、唐古特大 T2000P 型色谱工作站（上海天美科学仪器有限公司）； 黄