野葡萄根、猕猴桃根对高脂血症大鼠血脂等影响的实验研究_药学论文.docVIP

野葡萄根、猕猴桃根对高脂血症大鼠血脂等影响的实验研究_药学论文 野葡萄根、猕猴桃根对高脂血症大鼠血脂等影响的实验研究_药学论文 作者：吴大梅，万安霞，杨长福，张小容，樊同涛，徐中贵，俞昌琪 【摘要】 目的观察高脂血症大鼠应用野葡萄根、猕猴桃根治疗后血清中血脂的变化及一氧化氮(NO)和内皮素(ET)的水平，以探讨野葡萄根、猕猴桃根治疗高脂血症的机理。方法将55只雄性Wistar大鼠随机分为正常对照组，高脂模型组，野葡萄根、猕猴桃根组［8 g/（kg·d）］、复方葡猕汤组［11 g/（kg·d）］和辛伐他汀组［0.1 mg/（kg·d）］,每组11只，观察6周。实验中监测各组大鼠血脂及血清中NO和ET的含量。结果野葡萄根、猕猴桃根治疗组的血脂水平较高脂模型组明显降低( 0. 05) ；血清中NO明显升高，而ET含量明显降低(0.05，0.01)。结论野葡萄根、猕猴桃根能明显降低血脂，提高血清中的NO水平并降低ET的水平。NO和ET的变化可能是其保护高脂血症大鼠血管内皮功能的重要机制之一，证实野葡萄根、猕猴桃根在治疗高脂血症及高血压病过程中起到一定的作用。 【关键词】 野葡萄根；猕猴桃根；高脂血症；一氧化氮；内皮素 Abstract：ObjectiveTo evaluate the changes of serum lipids、 nitric oxide (NO) and endothelin (ET) after treatment with wild grape root and Kiwi root in rats fed with hypercholesterol emulsion. Methods55 male Wistar rats were randomly divided into control group ( n = 11 , normal diet) , hyperlipidemia group (n=11 , high-cholesterol emulsion ) and Chinese medicine group (every group n = 11, high-cholesterol emulsion plus corresponding Chinese medicine : (8g/kg/d , 11g/kg/d,). Western medicine group(n = 8 , high-cholesterol emulsion plus simvastatin). During 6 weeks of the experiment , serum lipids were compared among the 5 groups., the levels of NO and ET in serum were also measured. ResultsAfter 6 weeks of treatment with Ye Putao root and Kiwi root , the levels of serum lipids were all decreased as compared with hyperlipidemia group (all 0.05); the NO level in serum was increased significantly , while the ET level was markedly decreased as compared with hyperlipidemia group (P lt; 0. 05,0.01) . Conclusion Wild grape root、Kiwi root could effectively decrease the level of serum lipids, increase NO and decrease ET. The changes of NO and ET may play a role in the progress of hyperlipidemia. It confirmed that wild grape root and Kiwi root have certain effect on the hyperlipidemia and hypertension. 　　Key words：Wild grape root； Kiwi root ; Hyperlipidemia; Nitric oxide ; Endothelin 　　据调查，民间用野葡萄根、猕猴桃根、葛根治疗高脂血症及高血压病，并取得一定疗效。葛根为常用中药，具有扩血管、促进微循环作用，而野葡萄根、猕猴桃根为贵州苗药，它们在治疗过程中有