食管癌基因表达谱及其与细胞分化的相关性研究_医学论文.docVIP

食管癌基因表达谱及其与细胞分化的相关性研究_医学论文 食管癌基因表达谱及其与细胞分化的相关性研究_医学论文 作者：李沛，凌志强，杨洪艳，黄幼田，赵继敏，董子明 【关键词】 食管肿瘤 Study on gene expression profiles and their relation to cell differentiation in human esophageal squamous cell carcinoma 　　【Abstract】 AIM: To investigate the gene expression profiles and to screen the genes related to cell differentiation in primary esophageal cancer, which is helpful to search and locate cell differentiationassociated genes and deepen the understanding of the molecular mechanism of its tumourgenesis. METHODS: cDNA microarray was used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 cases of human esophageal squamous cell carcinoma (ESCC). The results were analyzed by bioinformatics. RESULTS: Among the 886 target genes, 110 (12.42%) genes were differentially expressed commonly at least twice in all 15 samples. There was a correlation between 16 (1.81%) genes and cell differentiation, of which 9 (1.02%) were upregulated and 7 (0.79%) downregulated. CONCLUSION: Many ESCCassociated genes were screened by highthroughput gene chip method validating their cellular functions will help to identify the key genes or pathway responsible for ESCC, which might be used as diagnostic markers and therapeutic targets for ESCC. 　　【Keywords】 esophageal neoplasms cDNA microarray gene expression profile cell differentiation 　　【摘要】 目的： 研究原发性食管癌基因表达谱，筛选与肿瘤细胞分化相关的基因，旨在寻找或定位食管癌分化相关基因，加深对其癌变分子机制的了解. 方法： 应用基因芯片技术对15例ESCC癌组织和正常组织的mRNA进行检测，并用生物信息学对检测结果进行分析. 结果： 在886条目的基因中有110（12.42%）条至少2次出现差异表达，与肿瘤分化直接相关的基因有16条（1.81%），其中表达上调的9条（1.02%），表达下调的7条（0.79%）. 结论： 高通量的基因芯片技术筛选出大量的ESCC相关基因，从中可分析出与肿瘤分化相关的基因. 对这些相关基因功能的验证将有助于找到ESCC发生、发展的关键基因或通路，并可作为ESCC诊断的指标和治疗的靶点. 　　【关键词】 食管肿瘤；基因芯片；基因表达谱；细胞分化 　　0引言 　　食管癌（esophageal carcinoma）是人类常见的恶性肿瘤之一. 全世界每年约30万死于食管癌，其中50%在我国，每年因食管癌死亡的人数约占我国全部恶性肿瘤死亡总数的1/4，严重危害人类健康［1］. 食管癌按病理学类型主要分为鳞状细胞癌和腺癌. 两者在病因学与病理学特征上存在明显差异，在中国主要以鳞状细胞癌为主. 细胞分化异常导致上皮细胞发生恶性转化，是食管鳞癌发生的重要机制之一［1］. 随着细胞分子肿瘤学的发展，食管鳞癌分化相关基因的筛选验证、基因表达调控机制的探讨已成为肿瘤领域的研究