嗜酸兼性异养菌Acidiphilium sp. DX1-1的分离、特征及其浸矿行为.pdfVIP

Trans. Nonferrous Met. Soc. China 23(2013) 1774−1782 Isolation, characterization of Acidiphilium sp. DX1-1 and ore bioleaching by this acidophilic mixotrophic organism Yan-fei ZHANG1,2 1,2 1,2 1 1,2 , An-an PENG , Yu YANG , Jian-she LIU , Guan-zhou QIU 1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha 410083, China Received 11 January 2013; accepted 5 June 2013 Abstract: The isolation and characterization of a subspecies of Acidiphilium that not only acts as an enhancer of other autotrophic acidophiles in bioleaching, but also has significant leaching capacity towards marmatite was described. Acidiphilium sp. DX1-1, a Gram-negative, motile, short rod-shaped bacterium that accumulates intracellular polyhydroxybutyrate, was isolated from the Dexing copper mine area in China. It is mesophilic and acidophilic with an optimum growth at 30 °C and pH 3.5. Phylogenetic analyses identify that it is a member of genus Acidiphilium and closely related to Acidiphilium cryptum and Acidiphilium multivorum. It is mixotrophic, utilizing organic substrates and a range of inorganic substrates, such as sulfur, ferric iron and a variety of sulfide minerals. Acidiphilium sp. DX1-1 is able to bioleach 40% of the zinc content of marmatite with the initial pH 3.5 within a month, which is even higher than that of A. ferrooxidans or the mixed culture with A. ferrooxidans at even lower pH. Key words: Acidiphilium sp.; bioleaching; marmatite; chalcopyrite; polyhydroxybutyrate; 16S rRNA